Dickneite G, Sedlacek H H, Timpl R, Seiler F R
Behring Inst Mitt. 1985 Dec(78):159-66.
Serum concentrations of two basement membrane proteins, laminin and collagen type IV, were studied in three spontaneously metastasising experimental tumors: 3LL Lewis Lung and B16 melanoma of the mouse and R3230 adenocarcinoma of the rat. In the Lewis Lung tumor the mass of the primary tumor or the degree of metastasis correlated well with the concentration of serum laminin. In B16 melanoma the level of serum laminin was only significantly raised when the tumor had metastasised to the lung. No correlation between tumor mass and serum laminin was found in the R3230 adenocarcinoma. The serum concentrations of collagen IV, however, were raised to a certain extent during primary tumor growth and metastasis. Immunofluorescent staining for laminin in the Lewis Lung tumor demonstrated positive reactions in the primary tumor and in metastases of the lung. The data indicate the possibility of using similar tests for clinical tumor diagnosis.
研究了三种自发转移的实验性肿瘤（小鼠的3LL Lewis肺癌和B16黑色素瘤以及大鼠的R3230腺癌）中两种基底膜蛋白（层粘连蛋白和IV型胶原蛋白）的血清浓度。在Lewis肺癌中，原发肿瘤的大小或转移程度与血清层粘连蛋白浓度密切相关。在B16黑色素瘤中，只有当肿瘤转移至肺部时，血清层粘连蛋白水平才会显著升高。在R3230腺癌中，未发现肿瘤大小与血清层粘连蛋白之间存在相关性。然而，在原发肿瘤生长和转移过程中，IV型胶原蛋白的血清浓度在一定程度上有所升高。对Lewis肺癌进行层粘连蛋白的免疫荧光染色显示，原发肿瘤和肺部转移灶均呈阳性反应。这些数据表明，有可能将类似的检测方法用于临床肿瘤诊断。
