Lenz S
Clin Obstet Gynaecol. 1985 Dec;12(4):785-98.
A technique for oocyte collection by percutaneous puncture guided by ultrasound is described. The equipment includes a sector scanner with a puncture transducer. A coarse needle (16 gauge) and a syringe constitute the aspiration device. The syringe provides the vacuum and serves as a trap for the follicular fluids. Follicular flushing is performed by syringes containing culture medium. The aspiration is carried out as an out-patient procedure under local anaesthesia after premedication. The spontaneously filled bladder acts as an ultrasonic window and the needle is introduced through the bladder into the ovarian follicles, guided by the screen display. The oocyte recovery rate per follicle exceeds 80% with experience, and the collection can be performed in women with abundant pelvic adhesions. The only complication to the procedure is a short-lasting haematuria, observed in 5% of the cases.
描述了一种在超声引导下经皮穿刺采集卵母细胞的技术。设备包括带有穿刺换能器的扇形扫描仪。一根粗针（16号）和一个注射器构成抽吸装置。注射器提供真空并用作卵泡液的收集器。用含有培养基的注射器进行卵泡冲洗。抽吸在术前用药后于局部麻醉下作为门诊手术进行。自然充盈的膀胱作为超声窗口，在屏幕显示的引导下，将针经膀胱插入卵巢卵泡。随着经验积累，每个卵泡的卵母细胞回收率超过80%，该采集方法可用于盆腔粘连严重的女性。该手术唯一的并发症是5%的病例中出现短暂性血尿。
