Lu Zhe, Yu Xiaohan, Li Pengjie, Wang Yiming, Deng Yeyun, Li Xin, Wang Chaodong, Yu Shun
Department of Neurobiology and National Clinical Research Center for Geriatric Diseases, Xuanwu Hospital of Capital Medical University, Beijing, China.
Department of Neurology, Xuanwu Hospital of Capital Medical University, Beijing, China.
Front Aging Neurosci. 2024 Jul 31;16:1437622. doi: 10.3389/fnagi.2024.1437622. eCollection 2024.
Oligomeric alpha-synuclein in red blood cells (RBC-o-α-Syn) has been shown to be increased in patients with Parkinson's disease (PD). However, factors that affect RBC-o-α-Syn levels remain to be elucidated. The aim of this study is to analyze the correlations between RBC-o-α-Syn levels and the age, sex and different clinical variables of patients with PD.
167 patients with PD and 119 healthy controls (HC) were enrolled in this study. The patients with PD were diagnosed based on the MDS clinical diagnostic criteria for PD. All participants were evaluated for their clinical characteristics. Western blot analysis was used to examine the molecular sizes of RBC-o-α-Syn. A newly established chemiluminescent immunoassay was used to measure RBC-o-α-Syn levels.
Higher RBC-o-α-Syn levels were detected in PD patients than in HC subjects. The receiver operating characteristic (ROC) curve indicated that a cut off value of 55.29 ng/mg discriminated well between PD patients and HC subjects, with a sensitivity of 67.66% (95% CI: 60.24-74.29%), a specificity of 88.24% (95% CI: 81.22-92.86%), and an area under the curve (AUC) of 0.857. The levels of RBC-o-α-Syn were higher in female than male patients ( = 0.033). For different subtypes, the levels of RBC-o-α-Syn were higher in the MIX subtype than the tremor-dominant (TD) PD. In addition, the levels of RBC-o-α-Syn were higher in patients with than without cognitive impairment ( = 0.016), and negatively correlated with Mini-Mental State Examination (MMSE) scores ( = -0.156, = 0.044).
Our study demonstrates that RBC-o-α-Syn levels in patients with PD are higher than those in HC subjects and affected by the sex and the severity of cognitive impairment.
红细胞中寡聚α-突触核蛋白（RBC-o-α-Syn）在帕金森病（PD）患者中已被证明有所增加。然而，影响RBC-o-α-Syn水平的因素仍有待阐明。本研究的目的是分析RBC-o-α-Syn水平与PD患者的年龄、性别及不同临床变量之间的相关性。
本研究纳入了167例PD患者和119例健康对照（HC）。PD患者根据MDS的PD临床诊断标准进行诊断。对所有参与者的临床特征进行评估。采用蛋白质免疫印迹分析检测RBC-o-α-Syn的分子大小。使用新建立的化学发光免疫分析法测量RBC-o-α-Syn水平。
PD患者的RBC-o-α-Syn水平高于HC受试者。受试者工作特征（ROC）曲线表明，55.29 ng/mg的截断值能很好地区分PD患者和HC受试者，灵敏度为67.66%（95%CI：60.24 - 74.29%），特异性为88.24%（95%CI：81.22 - 92.86%），曲线下面积（AUC）为0.857。女性患者的RBC-o-α-Syn水平高于男性患者（P = 0.033）。对于不同亚型，混合（MIX）亚型的RBC-o-α-Syn水平高于震颤为主型（TD）PD。此外，有认知障碍的患者的RBC-o-α-Syn水平高于无认知障碍的患者（P = 0.016），且与简易精神状态检查表（MMSE）评分呈负相关（r = -0.156，P = 0.044）。
我们的研究表明，PD患者的RBC-o-α-Syn水平高于HC受试者，且受性别和认知障碍严重程度的影响。
