抗体与猪透明带的反应性。

Antibody reactivity with porcine zona pellucida.

作者信息

Mori T, Kamada M, Hasebe H, Irahara M, Shivers A, Czuppon A B, Mettler L, Mathur S, Dunbar B S

出版信息

J Reprod Immunol. 1985 Dec;8(4):337-45. doi: 10.1016/0165-0378(85)90008-7.

Abstract

A comparative study on anti-zona antibody activities in the sera from clinically defined categories of patients registered at the WHO Reference Bank for Reproductive Immunology was performed at five different laboratories with different detection methods. Considerably higher incidences of positive reactions were detected by immunofluorescence on porcine zonae in infertile women (16.3%) than in control subjects (7.1%). A similar proportion of positives was found by radioimmuno-binding assay (RIBA) using porcine zona antigen preparation in the infertile group (13.0%) but not in the female control group (0%), giving an indication of the specificity of this test. It is noteworthy that high incidences of positives were observed by RIBA with sera from male subjects with unexplained sterility, vasectomy and aspermatogenesis. A test system of passive hemagglutination reaction (PHAR) using purified porcine zona substance as antigen gave a low but slightly higher incidence of positives in infertile (3.1%) than in control sera (0.9%). No positive reactions were observed with infertile and control sera by another PHAR or by radioimmunoassay using an antigen preparation common to the two test systems. Anti-zona activities in these sera were therefore seen to vary, depending largely upon the detection systems and the antigen preparations.

摘要

在世卫组织生殖免疫学参考库登记的不同临床类型患者血清中的抗透明带抗体活性，由五个不同实验室采用不同检测方法进行了一项比较研究。通过对猪透明带进行免疫荧光检测发现，不育女性的阳性反应发生率（16.3%）显著高于对照组（7.1%）。在不育组中，采用猪透明带抗原制剂的放射免疫结合试验（RIBA）检测到的阳性比例与之相似（13.0%），而在女性对照组中未检测到阳性（0%），这表明该检测具有特异性。值得注意的是，采用RIBA检测不明原因不育、输精管结扎和无精子症男性受试者的血清时，也观察到了较高的阳性发生率。以纯化猪透明带物质为抗原的被动血凝反应（PHAR）检测系统，不育血清的阳性发生率较低但略高于对照血清（不育血清为3.1%，对照血清为0.9%）。采用另一种PHAR或使用两种检测系统通用抗原制剂的放射免疫测定法，对不育血清和对照血清检测均未观察到阳性反应。因此，这些血清中的抗透明带活性因检测系统和抗原制剂的不同而有所差异。