Zwara Agata, Hellmann Andrzej, Czapiewska Monika, Korczynska Justyna, Sztendel Alicja, Mika Adriana
Department of Environmental Analytics, Faculty of Chemistry, University of Gdansk, Gdańsk, Poland.
Department of General, Endocrine and Transplant Surgery, Faculty of Medicine, Medical University of Gdansk, Gdańsk, Poland.
Endocrine. 2025 Jan;87(1):273-280. doi: 10.1007/s12020-024-03993-z. Epub 2024 Aug 15.
Over the years we observed changes in the metabolism of glucose, amino acids, fatty acids (FA) and nucleotides in cancer cells in order to maintain their viability and proliferate. Moreover, as the latest data show, cancer also forces a complete change in the behavior of other tissues. For instance, fat-filled adipocytes are often found in the vicinity of invasive solid human tumors. We investigated the effects of papillary thyroid carcinoma (PTC) on the lipid metabolism of healthy tissue distant from the tumor.
Thyroid tissue was collected from female patients immediately after surgical removal of the entire thyroid gland. Blood samples were collected from PTC patients and healthy volunteers. Real-time PCR assays were performed to analyze the expression of lipogenic genes and a broad panel of FA was determined using the gas chromatography-mass spectrometry method.
The concentration of lipids was higher in paratumor tissue than in healthy thyroid tissue (p = 0.005). The lipogenic genes tested were significantly increased in paratumor tissue compared to healthy tissue, especially enzymes related to the synthesis of very long-chain saturated and polyunsaturated FAs (VLCSFAs and PUFAs, respectively) (p < 0.001). The FA profile also showed increased levels of C22-C26, VLCSFAs and almost all PUFAs in paratumor tissue (p < 0.05).
Our study suggests that a restructuring of lipid metabolism occurs in the adjacent healthy thyroid gland and that the metabolism of VLCSFAs and PUFAs is higher in the paratumor tissue than in the distant tissue of the healthy thyroid gland.
多年来，我们观察到癌细胞中葡萄糖、氨基酸、脂肪酸（FA）和核苷酸的代谢发生变化，以维持其生存能力并进行增殖。此外，最新数据表明，癌症还会迫使其他组织的行为发生彻底改变。例如，在侵袭性实体人类肿瘤附近经常发现充满脂肪的脂肪细胞。我们研究了甲状腺乳头状癌（PTC）对远离肿瘤的健康组织脂质代谢的影响。
在手术切除整个甲状腺后，立即从女性患者身上采集甲状腺组织。从PTC患者和健康志愿者身上采集血样。进行实时PCR分析以检测生脂基因的表达，并使用气相色谱 - 质谱法测定多种脂肪酸。
肿瘤旁组织中的脂质浓度高于健康甲状腺组织（p = 0.005）。与健康组织相比，肿瘤旁组织中检测的生脂基因显著增加，尤其是与极长链饱和脂肪酸和多不饱和脂肪酸（分别为VLCSFAs和PUFAs）合成相关的酶（p < 0.001）。脂肪酸谱还显示肿瘤旁组织中C22 - C26、VLCSFAs和几乎所有PUFAs的水平升高（p < 0.05）。
我们的研究表明，相邻的健康甲状腺腺体会发生脂质代谢重构，并且肿瘤旁组织中VLCSFAs和PUFAs的代谢高于健康甲状腺的远处组织。
