Moltz J H, McDonald J K
Peptides. 1985 Nov-Dec;6(6):1155-9. doi: 10.1016/0196-9781(85)90443-7.
Neuropeptide Y (NPY) was tested for an ability to directly influence the release of insulin using an in vitro isolated rat pancreatic islet system. NPY, at doses ranging from 100 pg/ml to 1 microgram/ml, had no significant effect on the basal release (5.5 mM glucose) of insulin. However, NPY treatment resulted in a significant, dose-dependent (1 ng/ml to 1 microgram/ml) inhibition of glucose-stimulated (11 mM) insulin release. When tested in a perfused rat pancreas preparation in situ, NPY administration led to a marked inhibition of both basal and stimulated insulin release followed by a postinhibitory rebound which exceeded the control insulin levels by 3-fold. In contrast, the intracerebroventricular (ICV) microinjection of NPY (5 micrograms) produced a significant but delayed (30 min) elevation of circulating insulin. It is therefore suggested that the direct action of NPY on insulin release is inhibitory while the central action of NPY indirectly results in an increase in plasma insulin. Thus, NPY may be added to the growing list of peptidergic agents which may affect the endocrine pancreas by acting as neurotransmitters and/or neuromodulators.
利用体外分离的大鼠胰岛系统，检测了神经肽Y（NPY）直接影响胰岛素释放的能力。NPY剂量在100 pg/ml至1微克/毫升范围内时，对胰岛素的基础释放（5.5 mM葡萄糖）无显著影响。然而，NPY处理导致对葡萄糖刺激（11 mM）的胰岛素释放有显著的、剂量依赖性（1 ng/ml至1微克/毫升）的抑制作用。在原位灌流大鼠胰腺制剂中进行测试时，给予NPY导致基础和刺激的胰岛素释放均受到显著抑制，随后出现抑制后反弹，超过对照胰岛素水平3倍。相比之下，脑室内（ICV）微量注射NPY（5微克）使循环胰岛素显著升高，但有延迟（30分钟）。因此，提示NPY对胰岛素释放的直接作用是抑制性的，而NPY的中枢作用间接导致血浆胰岛素增加。因此，NPY可能被添加到越来越多的肽能药物名单中，这些药物可能通过作为神经递质和/或神经调节剂来影响内分泌胰腺。
