Proteomics Center of Excellence, Northwestern University, Evanston, Illinois 60208, United States.
Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States.
J Proteome Res. 2024 Sep 6;23(9):3944-3957. doi: 10.1021/acs.jproteome.4c00285. Epub 2024 Aug 15.
Solid organ transplant recipients with immunosuppressant regimens to prevent rejection are less able to mount effective immune responses to pathogenic infection. Here, we apply a recently reported mass spectrometry-based serological approach known as Ig-MS to characterize immune responses against infection with SARS-CoV-2 in cohorts of transplant recipients and immunocompetent controls, both at a single early time point following COVID-19 diagnosis as well as over the course of one-month postdiagnosis. We found that the antibody repertoires generated by transplant recipients against SARS-CoV-2 do not differ significantly compared to immunocompetent individuals with regard to repertoire titer, clonality, or glycan composition. Importantly, our study is the first to characterize the evolution of antibody glycan profiles in transplant recipients with COVID-19 disease, presenting evidence that the evolution of glycan composition in these immunocompromised individuals is similar to that in immunocompetent people.
接受免疫抑制方案以预防排斥反应的实体器官移植受者，其针对病原体感染产生有效免疫反应的能力降低。在这里，我们应用一种最近报道的基于质谱的血清学方法（称为 Ig-MS），在 COVID-19 诊断后单个早期时间点以及诊断后一个月的时间内，对移植受者和免疫功能正常的对照者队列进行 SARS-CoV-2 感染的免疫反应进行特征描述。我们发现，与免疫功能正常的个体相比，移植受者针对 SARS-CoV-2 产生的抗体库在库容量、克隆性或聚糖组成方面没有显著差异。重要的是，我们的研究首次对 COVID-19 疾病中的移植受者的抗体聚糖谱的演变进行了特征描述，提供了证据表明，这些免疫功能低下个体的聚糖组成的演变与免疫功能正常的个体相似。
