School of Environmental and Resource Sciences, Fujian Normal University, Fujian Key Laboratory of Pollution Control and Resource Reuse, Fuzhou, Fujian Province 350117, PR China.
Environmental Contaminants Group, Future Industries Institute, University of South Australia, Mawson Lakes, SA 5095, Australia.
J Hazard Mater. 2024 Oct 5;478:135503. doi: 10.1016/j.jhazmat.2024.135503. Epub 2024 Aug 13.
Green synthesized iron/manganese nanoparticles (Fe/Mn NPs), acted as an exogenous promoter to enhance the lignin-degrading bacteria Comamonas testosteroni FJ17 resulting in more efficient removal of bisphenol A (BPA). Batch experiments demonstrated that removal efficiency of BPA via cells at a BPA concentration of 10 mg·L increased by 20.9 % when exposed to 100 mg·L Fe/Mn NPs after 48 h (93.63 %) relative to an unexposed control group (72.70 %). TEM and 3D-EEM analysis confirmed that the cell membrane thickness increased from 47 to 80 nm under Fe/Mn NPs exposure, and the TB-EPS secretion was promoted. Meanwhile, Fe/Mn NPs facilitated greater electron transfer capacity of c-cytochrome (0.55 V reduction peak) and an unknown cytochrome substance (0.7 V oxidation peak) on the surface of cells. Studies of the effect of Fe/Mn NPs on both the growth and activity of laccase cells showed that both biomass and laccase secretion increased significantly during the logarithmic growth period (6-36 h). LC-MS analysis and toxicity assessment indicated that Fe/Mn NPs decreased the degradation time of BPA and efficiently reduced the toxicity of its by-products. Transcriptomic analysis revealed 315 up-regulation of the key genes associated with energy supply, membrane translocation, and metabolic pathways upon exposure to Fe/Mn NPs. Such as MFS transporter (2.27-fold), diguanylate cyclase (1.76-fold) and protocatechuate-3,4-dioxygenase (1.62-fold). Overall, Fe/Mn NPs accelerated proliferation by enhancing metabolic capacity and nutrient transport processes, which serves to improve the efficiency of BPA removal.
绿色合成的铁/锰纳米粒子（Fe/Mn NPs）作为一种外源促进剂，可增强木质素降解菌考氏 Comamonas testosteroni FJ17，从而更有效地去除双酚 A（BPA）。批处理实验表明，当在 48 小时内暴露于 100mg·L 的 Fe/Mn NPs 时，细胞在 10mg·L 的 BPA 浓度下对 BPA 的去除效率提高了 20.9%（93.63%），与未暴露对照组（72.70%）相比。TEM 和 3D-EEM 分析证实，在 Fe/Mn NPs 暴露下，细胞膜厚度从 47nm 增加到 80nm，并且 TB-EPS 分泌得到促进。同时，Fe/Mn NPs 促进了细胞表面 c-细胞色素（0.55V 还原峰）和未知细胞色素物质（0.7V 氧化峰）的电子转移能力。研究 Fe/Mn NPs 对漆酶细胞的生长和活性的影响表明，在对数生长期（6-36 小时），生物量和漆酶分泌均显著增加。LC-MS 分析和毒性评估表明，Fe/Mn NPs 缩短了 BPA 的降解时间，有效降低了其副产物的毒性。转录组分析表明，暴露于 Fe/Mn NPs 后，与能量供应、膜转运和代谢途径相关的 315 个关键基因上调。例如 MFS 转运蛋白（2.27 倍）、双鸟苷酸环化酶（1.76 倍）和原儿茶酸-3,4-双加氧酶（1.62 倍）。总的来说，Fe/Mn NPs 通过增强代谢能力和营养物质运输过程来加速增殖，从而提高 BPA 的去除效率。
