Pilleri G, Faa G, Liguori C, Goffi C, Lai E
Ric Clin Lab. 1985 Oct-Dec;15(4):337-42. doi: 10.1007/BF03029148.
Delta antigen (delta-Ag), hepatitis B surface antigen (HBsAg) and hepatitis B core antigen (HBcAg) were examined by immunofluorescence and immunoperoxidase staining in 106 deparaffinized liver biopsy samples from HBsAg-positive patients with acute and chronic hepatitis. The delta-Ag was present in 15 cases (14%), with nuclear positivity varying greatly in intensity and prevalence. Patients with chronic hepatitis associated with delta infection had a histological picture characterized by foci of intralobular inflammation, many apoptotic bodies and shrunken hepatocytes with no satellite signs of inflammation. The histological pattern of delta antigen-positive acute hepatitis was characterized by the presence of a large number of intralobular apoptotic bodies. The inflammatory reaction mainly involved the portal tracts with piecemeal necrosis, but without collagen production. In the same cases the pattern of expression of HBsAg and HBcAg was unusual: in three cases HBcAg and HBsAg were concomitantly present, whereas in one case none of the hepatitis B virus markers was detectable.
采用免疫荧光和免疫过氧化物酶染色法，对106例HBsAg阳性的急慢性肝炎患者的石蜡包埋肝活检样本进行了丁型抗原（delta-Ag）、乙型肝炎表面抗原（HBsAg）和乙型肝炎核心抗原（HBcAg）检测。15例（14%）检测到丁型抗原，其核阳性强度和发生率差异很大。丁型肝炎感染相关的慢性肝炎患者的组织学表现为小叶内炎症灶、许多凋亡小体和萎缩的肝细胞，无炎症卫星灶。丁型抗原阳性急性肝炎的组织学特征是大量小叶内凋亡小体的存在。炎症反应主要累及汇管区并伴有碎片状坏死，但无胶原生成。在同一病例中，HBsAg和HBcAg的表达模式异常：3例同时存在HBcAg和HBsAg，而1例未检测到任何乙肝病毒标志物。
