São Paulo State University (UNESP), Department of Physics and Chemistry, Faculty of Engineering, Ilha Solteira, SP, Brazil.
Plant Resources Study Group, Natural Resources Program, Center for Natural Resources Study (CERNA), Mato Grosso do Sul State University (UEMS), Dourados, MS, Brazil; Optics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil.
Chemosphere. 2024 Sep;364:143080. doi: 10.1016/j.chemosphere.2024.143080. Epub 2024 Aug 13.
Nanotechnology has brought significant advancements to agriculture through the development of engineered nanomaterials (ENPs). Silver nanoparticles (AgNPs) capped with polysaccharides have been applied in agricultural diagnostics, crop pest management, and seed priming. Hyaluronic acid (HA), a natural polysaccharide with bactericidal properties, has been considered a growth regulator for plant tissues and an inducer of systemic resistance against plant diseases. Additionally, HA has been employed as a stabilizing agent for AgNPs. This study investigated the synthesis and effects of hyaluronic acid-stabilized silver nanoparticles (HA-AgNPs) as a seed priming agent on lettuce (Lactuca sativa L.) seed germination. HA-AgNPs were characterized using several techniques, exhibiting spherical morphology and good colloidal stability. Germination assays conducted with 0.1, 0.04, and 0.02 g/L of HA-AgNPs showed a concentration-dependent reduction in seed germination. Conversely, lower concentrations of HA-AgNPs significantly increased germination rates, survival, tolerance indices, and seed water absorption compared to silver ions (Ag). SEM/EDS indicated more significant potential for HA-AgNPs internalization compared to Ag. Therefore, these findings are innovative and open new avenues for understanding the impact of Ag and HA-AgNPs on seed germination.
纳米技术通过工程纳米材料（ENPs）的发展为农业带来了重大进步。用多糖包覆的银纳米粒子（AgNPs）已应用于农业诊断、作物害虫管理和种子引发。具有杀菌特性的天然多糖透明质酸（HA）已被认为是植物组织的生长调节剂和植物疾病系统抗性的诱导剂。此外，HA 还被用作 AgNPs 的稳定剂。本研究探讨了作为种子引发剂的透明质酸稳定的银纳米粒子（HA-AgNPs）对生菜（Lactuca sativa L.）种子萌发的合成和影响。使用多种技术对 HA-AgNPs 进行了表征，结果表明其具有球形形态和良好的胶体稳定性。用 0.1、0.04 和 0.02 g/L 的 HA-AgNPs 进行的萌发试验表明，种子萌发率随浓度的降低而降低。相反，与银离子（Ag）相比，较低浓度的 HA-AgNPs 显著提高了发芽率、成活率、耐盐指数和种子吸水率。SEM/EDS 表明，与 Ag 相比，HA-AgNPs 的内化潜力更大。因此，这些发现具有创新性，为理解 Ag 和 HA-AgNPs 对种子萌发的影响开辟了新途径。
