Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
J Proteomics. 2024 Sep 30;308:105280. doi: 10.1016/j.jprot.2024.105280. Epub 2024 Aug 13.
Metabolomics significantly impacts drug discovery and precise disease management. This study meticulously assesses the metabolite profiles of cells treated with Crocin, Dexamethasone, and mesenchymal stem cells (MSCs) under oxidative stress induced by 2-chloroethyl ethyl sulfide (CEES). Gas chromatography/mass spectrometry (GC/MS) analysis unequivocally identified substantial changes in 37 metabolites across the treated groups. Notably, pronounced alterations were observed in pathways associated with aminoacyl-tRNA biosynthesis and the metabolism of aspartate, serine, proline, and glutamate. These findings demonstrate the potent capacity of the analyzed treatments to effectively reduce inflammation, mitigate reactive oxygen species production, and enhance cell survival rates. SIGNIFICANCE.
代谢组学对药物发现和精确疾病管理有重大影响。本研究细致评估了细胞在 2-氯乙基乙基硫醚（CEES）诱导的氧化应激下，经藏红花酸、地塞米松和间充质干细胞（MSCs）处理后的代谢产物谱。气相色谱/质谱（GC/MS）分析明确鉴定出处理组中 37 种代谢物发生了显著变化。值得注意的是，与氨酰-tRNA 生物合成和天冬氨酸、丝氨酸、脯氨酸和谷氨酸代谢相关的途径发生了明显改变。这些发现表明所分析的治疗方法具有强大的能力，可以有效减轻炎症、减少活性氧的产生并提高细胞存活率。意义重大。
