Peter Debye Institute for Soft Matter Physics, University of Leipzig, Leipzig, Germany.
Istituto di Fotonica e Nanotecnologie (IFN)-CNR, Milan, Italy.
Methods Mol Biol. 2024;2828:11-21. doi: 10.1007/978-1-0716-4023-4_2.
The extracellular environment plays a crucial role in many physiological and pathological processes involving cell motility, such as metastatic invasion in cancer development, by heavily impacting the migration strategies adopted by the cells. The study of how mechanical constraints affect the dynamics of cell migration may be relevant to gain more insight into such processes, and it may prove to be a powerful tool in the hands of biologists. In this chapter, we describe the methods used to investigate the ability of neoplastic cells to migrate through narrowing, rigid microstructures upon chemoattractant stimulation.
细胞外环境在许多涉及细胞运动的生理和病理过程中起着至关重要的作用，例如癌症发展中的转移侵袭，它会严重影响细胞采用的迁移策略。研究机械约束如何影响细胞迁移的动力学可能有助于更深入地了解这些过程，并且它可能成为生物学家手中的有力工具。在本章中，我们描述了用于研究肿瘤细胞在趋化因子刺激下通过狭窄、刚性微结构迁移能力的方法。
