Institute of Physics and Astronomy, University of Potsdam, Potsdam, Germany.
Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa, Japan.
Methods Mol Biol. 2024;2828:79-85. doi: 10.1007/978-1-0716-4023-4_8.
Bacteria can propel themselves by rotating a flagellum or a flagellar bundle. To image this thin structure in motile bacteria, the flagella can be vitally stained with fluorophores. This chapter describes a flagellar staining protocol with the additional possibility of visualizing the cell body. It offers the opportunity to track conformational changes of flagella and simultaneously track the positions of the cell bodies. The additional use of a filter increases the number of motile cells and improves the signal-to-noise ratio of images. The flagellar staining requires a prior introduction of a surface-exposed cysteine, which is not covered in this chapter.
细菌可以通过旋转鞭毛或鞭毛束来推动自身。为了在运动细菌中对这种细结构进行成像，可以用荧光染料对鞭毛进行活体染色。本章描述了一种鞭毛染色方案，该方案还具有可视化细胞体的额外可能性。它提供了跟踪鞭毛构象变化的机会，同时还可以跟踪细胞体的位置。额外使用过滤器可以增加运动细胞的数量并提高图像的信噪比。该鞭毛染色需要预先引入一个暴露在表面的半胱氨酸，这不在本章介绍范围内。
