Mayfield C I, Inniss W E
Can J Microbiol. 1977 Sep;23(9):1311-3. doi: 10.1139/m77-198.
A simple modification of Gray's flagellar staining procedure is described. It can be used on air-dried smears or directly on wet mounts of motile bacteria. The stained bacterial flagella can be observed with phase-contrast or bright-field optics. No rigorous cleaning of slides, counterstains, or any washing procedures are required with the staining method, making it very suitable for routine examinations.
本文描述了对格雷氏鞭毛染色程序的一种简单改进。它可用于风干涂片或直接用于活动细菌的湿涂片。染色后的细菌鞭毛可用相差显微镜或明视野光学显微镜观察。该染色方法无需对玻片进行严格清洗、使用复染剂或任何洗涤程序,非常适合常规检查。