Vaccine & Infectious Disease Organization (VIDO), Saskatoon, Saskatchewan, Canada.
Biological Informatics Center of Excellence, Agriculture and Agri-Food Canada, Saskatoon, Saskatchewan, Canada.
Future Microbiol. 2024 Sep;19(13):1129-1144. doi: 10.1080/17460913.2024.2359879. Epub 2024 Aug 16.
To compare the microbial communities inside hemodialysis catheters from symptomatic and asymptomatic patients to determine their differences. Catheters ( = 41) were removed from patients in the Saskatchewan Health Authority over an 18-month period. The catheter section inside the body was flushed and the contents were evaluated using culture-dependent and culture-independent analysis. All catheters were colonized by bacteria, with considerable overlap between groups based on microbial communities and the individual species detected. More Gram-negative species were detected by sequencing, whereas predominantly Gram-positive strains were cultured. Antibiotic resistance and biofilm formation was widespread and not correlated with either catheter group. Common pathogens were detected in each set of catheters, therefore predicting infections based on the microbiology is difficult.
比较有症状和无症状患者血液透析导管内的微生物群落，以确定它们的差异。在 18 个月的时间里，从萨斯喀彻温省卫生局的患者身上取出了导管（=41）。冲洗导管体内部分，并使用依赖培养和非依赖培养分析评估内容物。所有导管都被细菌定植，基于微生物群落和检测到的单个物种，两组之间有很大的重叠。通过测序检测到更多的革兰氏阴性物种，而培养的则主要是革兰氏阳性菌株。抗生素耐药性和生物膜形成很普遍，与导管组无关。在每一组导管中都检测到了常见的病原体，因此根据微生物学预测感染是困难的。
