Capital Institute of Pediatrics, Beijing 100020, PR China.
Beijing Obstetrics and Gynecology Hospital, Capital Medical University & Beijing Maternal and Child Health Care Hospital, Beijing 100026, PR China.
J Med Microbiol. 2024 Aug;73(8). doi: 10.1099/jmm.0.001862.
spp. are important bacteria that colonize the human intestine, especially in preterm infants; they can induce local and systemic disease under specific circumstances, including inflammatory bowel disease, necrotizing enterocolitis and colorectal cancer. spp. colonized in the intestine of the neonates in the neonatal intensive care unit (NICU) may be associated with disease and antibiotic resistance, which will be hazardous to the children. Our aim was to know about the prevalence, antimicrobial resistance and genome characteristics of spp. in neonate carriers. Genome sequencing and analysis, and antimicrobial susceptibility testing were mainly performed in this study. The isolation rates of spp. strains were 3.7% (16/436) in 2014 and 4.3% (18/420) in 2021. Cases with intestinal-colonized spp. were mainly infants with low birth weights or those with pneumonia or hyperbilirubinemia. According to the core-pan genomic analysis, 34 stains showed gene polymorphism and a sequence type (ST) of an emerging high-risk clone (ST11). Eight strains (23.5%) were found to be resistant to 2 or more antibiotics, and 46 genes/gene families along with nine plasmids were identified that conferred resistance to antibiotics. In particular, the two strains were multidrug-resistant. Strain A1256 that is related to was uncommon, carrying two plasmids similar to IncFII and IncX3 that included five antibiotic resistance genes. The prevention and control of neonatal spp. colonization in the NICU should be strengthened by paying increased attention to preventing antimicrobial resistance in neonates.
spp. 是定植于人类肠道的重要细菌，尤其在早产儿中更为常见；在特定情况下，如炎症性肠病、坏死性小肠结肠炎和结直肠癌，它们可能引发局部和全身疾病。在新生儿重症监护病房（NICU）中定植于新生儿肠道的 spp. 可能与疾病和抗生素耐药性有关，这对儿童是有危害的。我们的目的是了解新生儿携带者 spp. 的流行率、抗生素耐药性和基因组特征。本研究主要进行了基因组测序和分析以及抗生素药敏试验。2014 年和 2021 年， spp. 菌株的分离率分别为 3.7%（16/436）和 4.3%（18/420）。肠道定植 spp. 的病例主要是低出生体重儿或患有肺炎或高胆红素血症的婴儿。根据核心-泛基因组分析，34 株显示出基因多态性和一种新兴高危克隆（ST11）的序列类型（ST）。8 株（23.5%）对 2 种或更多种抗生素耐药，共鉴定出 46 个基因/基因家族和 9 个质粒，这些基因/基因家族和质粒赋予了对抗生素的耐药性。特别是，两株为多重耐药株。菌株 A1256 与 相关，携带两个与 IncFII 和 IncX3 相似的质粒，其中包含 5 个抗生素耐药基因。应加强 NICU 中新生儿 spp. 定植的预防和控制，以增加对新生儿抗生素耐药性的关注。
