Department of Pediatrics, Shandong Provincial Hospital, Shandong University, Jinan, Shandong, China.
Department of Pediatrics, Binzhou Medical University Hospital, Binzhou, Shandong, China.
PLoS One. 2024 Aug 16;19(8):e0307779. doi: 10.1371/journal.pone.0307779. eCollection 2024.
The main pathological change of myocarditis is an inflammatory injury of cardiomyocytes. Long noncoding RNAs (lncRNAs) are closely related to inflammation, and our previous study showed that differential expression of lncRNAs is associated with myocarditis. This study aimed to investigate the impact of lncRNAs on the onset of myocarditis.
RNA expression was measured by quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Lipopolysaccharide (LPS) was used to induce inflammation in human cardiomyocytes (HCMs). The expression of inflammatory cytokines and myocardial injury markers was detected by enzyme-linked immunosorbent assay (ELISA) and RT-qPCR. Cell viability and apoptosis were measured by the cell counting kit-8 assay and flow cytometry. The binding force between lncRNA NONHSAT122636.2 and microRNA miRNA-2110 was detected using the dual-luciferase assay.
NONHSAT122636.2 was dynamically expressed in patients with myocarditis and negatively correlated with inflammation severity. The overexpression of NONHSAT122636.2 improved inflammatory injury in LPS-stimulated HCMs. The study observed that there was a weak binding force between NONHSAT122636.2 and miR-2110.
NONHSAT122636.2 attenuates myocardial inflammation and apoptosis in myocarditis. Additionally, its expression decreases in the peripheral blood of children suffering from myocarditis and in patients who are diagnosed for the first time showing higher diagnostic sensitivity and specificity. This decrease is negatively correlated with the degree of inflammation. Overall, the study suggests that NONHSAT122636.2 can be exploited as a potential diagnostic biomarker for pediatric myocarditis.
心肌炎的主要病变是心肌细胞的炎症损伤。长链非编码 RNA（lncRNA）与炎症密切相关，我们之前的研究表明，lncRNA 的差异表达与心肌炎有关。本研究旨在探讨 lncRNA 对心肌炎发病的影响。
采用实时定量逆转录聚合酶链反应（RT-qPCR）检测 RNA 表达。脂多糖（LPS）用于诱导人心肌细胞（HCM）炎症。酶联免疫吸附试验（ELISA）和 RT-qPCR 检测炎症细胞因子和心肌损伤标志物的表达。细胞计数试剂盒-8 法和流式细胞术检测细胞活力和细胞凋亡。双荧光素酶报告基因检测 lncRNA NONHSAT122636.2 与 microRNA miRNA-2110 的结合力。
NONHSAT122636.2 在心肌炎患者中呈动态表达，与炎症严重程度呈负相关。NONHSAT122636.2 的过表达改善了 LPS 刺激的 HCM 中的炎症损伤。研究观察到 NONHSAT122636.2 与 miR-2110 之间存在弱结合力。
NONHSAT122636.2 减轻心肌炎中的心肌炎症和细胞凋亡。此外，在患有心肌炎的儿童和首次诊断的患者的外周血中，其表达降低，具有较高的诊断敏感性和特异性。这种降低与炎症程度呈负相关。总体而言，该研究表明 NONHSAT122636.2 可作为儿科心肌炎的潜在诊断生物标志物。
