School of Materials Science and Engineering, Central South University, Changsha, Hunan 410083, China.
Furong Laboratory, Central South University, Changsha, Hunan 410008, China.
Sci Adv. 2024 Aug 16;10(33):eado2741. doi: 10.1126/sciadv.ado2741.
The standard of clinical care of most malignant solid cancers is surgery, followed by postsurgical adjuvant therapy, but microtumor lesions left behind after surgery and invisible distant metastases are the major reasons for treatment failure. Here, we report an integrated strategy combining surface-enhanced Raman spectroscopy (SERS) surgical navigation with postsurgical immunotherapy elicited by near-infrared II photothermal treatment and programmed death-1 antibody. The SERS surgical navigation is principally based on the multifunctional optical probes (namely, MATRA probes) integrating with T-weighted magnetic resonance (MR) imaging, photothermal effect and Raman spectroscopic detection. We demonstrate in a 4T1 breast tumor mouse model that the pre-surgical MR/SERS dual-modal imaging is capable of providing comprehensive tumor information, and intraoperative SERS detection allows accurately delineating the tumor margins and guiding the surgical resection in real time with the least residual microscopic foci. We verify that the postsurgical immunotherapy effectively eradicates those local microtumor lesions and invisible distant metastases, greatly inhibiting the postsurgical cancer recurrence and distant metastasis.
大多数恶性实体瘤的临床治疗标准是手术，然后是术后辅助治疗，但手术遗留的微小肿瘤病灶和不可见的远处转移是治疗失败的主要原因。在这里，我们报告了一种综合策略，将表面增强拉曼光谱（SERS）手术导航与术后免疫治疗相结合，通过近红外 II 光热治疗和程序性死亡-1 抗体引发。SERS 手术导航主要基于多功能光学探针（即 MATRA 探针），整合了 T 加权磁共振（MR）成像、光热效应和拉曼光谱检测。我们在 4T1 乳腺癌小鼠模型中证明，术前 MR/SERS 双模成像能够提供全面的肿瘤信息，术中 SERS 检测能够实时准确地描绘肿瘤边界，并引导手术切除，最大限度地减少残留的微观焦点。我们验证了术后免疫治疗能够有效地消除局部微小肿瘤病灶和不可见的远处转移，大大抑制了术后癌症复发和远处转移。
