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一种用于准确检测食品中过氧苯甲酰的双反应位点荧光探针。

A dual-reaction sites fluorescent probe for accurate detection of benzoyl peroxide in food.

机构信息

Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, School of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China.

Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control, School of Light Industry and Food Engineering, Guangxi University, Nanning 530004, China..

出版信息

Food Chem. 2024 Dec 15;461:140822. doi: 10.1016/j.foodchem.2024.140822. Epub 2024 Aug 10.

DOI:10.1016/j.foodchem.2024.140822
PMID:39151345
Abstract

Benzoyl peroxide (BPO) is widely used as a whitening agent in flour, but excessive intake of BPO will severely endanger human health. To quickly and accurately detect BPO on-site, we have rationally designed a novel fluorescent probe PTPY-BE with dual-reaction sites. PTPY-BE underwent a specific cascade reaction with BPO to achieve high-contrast fluorescence turn-on response along with significant achromic reaction. The probe has high sensitivity, excellent selectivity, strong anti-interference ability and low detection limit (LOD = 0.83 mg·kg) for BPO. Furthermore, a portable detection platform was fabricated, which offers the portability and color visualization of traditional test strips and the color recognition of a smart device, enabling on-site visualization and quantitative detection of BPO. This platform has been successfully used to determine BPO in real food samples with good recoveries (93.59% - 107.13%). Therefore, this platform possessed great prospect and potential application for the determination of BPO in food.

摘要

过氧化苯甲酰(BPO)作为面粉增白剂被广泛使用,但过量摄入 BPO 会严重危害人体健康。为了快速准确地现场检测 BPO,我们合理设计了一种具有双反应位点的新型荧光探针 PTPY-BE。PTPY-BE 与 BPO 发生特异性级联反应,实现高对比度荧光开启响应,同时发生显著消色反应。该探针对 BPO 具有高灵敏度、优异选择性、强抗干扰能力和低检测限(LOD=0.83mg·kg)。此外,还构建了一种便携式检测平台,该平台兼具传统测试条的便携性和颜色可视化以及智能设备的颜色识别功能,可实现 BPO 的现场可视化和定量检测。该平台已成功用于实际食品样品中 BPO 的测定,回收率良好(93.59%-107.13%)。因此,该平台在食品中 BPO 的测定方面具有广阔的前景和潜在应用。

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