College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China.
College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China.
Genomics. 2024 Sep;116(5):110920. doi: 10.1016/j.ygeno.2024.110920. Epub 2024 Aug 14.
Follicles are fundamental units of the ovary, regulated intricately during development. Exosomes and ovarian granulosa cells (OGCs) play pivotal roles in follicular development, yet the regulatory mechanisms governing exosomes remain elusive.
High-throughput sequencing was employed to evaluate the complete transcript expression profiles of six samples (three porcine ovarian granulosa cells-exosome co-culture samples (GCE) and three porcine ovarian granulosa cells (POGCs) samples). Differential expression analysis revealed 924 lncRNAs, 35 circRNAs, 49 miRNAs, and 9823 mRNAs in the GCE group. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated enrichment of differentially expressed transcripts in pathways related to cell proliferation and apoptosis. Furthermore, a ceRNA regulatory network comprising 43 lncRNAs, 6 circRNAs, 11 miRNAs, and 126 mRNAs was constructed based on intergene co-expression correlations. Seven miRNAs associated with cell proliferation and apoptosis regulation were identified within this network, encompassing 92 subnet pairs as candidate genes for further exploration of exosome regulatory mechanisms. Additionally, preliminary verification at the cellular level demonstrated that exosomal miR-200b enhances the viability of POGCs.
Transcriptome analysis unveiled a pivotal candidate ceRNA network potentially implicated in exosome-mediated regulation of granulosa cell proliferation and apoptosis, thereby influencing porcine follicular development. These findings offer insights into the molecular mechanisms of follicular fluid exosome regulation, encompassing both coding and non-coding RNA perspectives.
卵泡是卵巢的基本单位，在发育过程中受到精细调控。外泌体和卵巢颗粒细胞（OGC）在卵泡发育中发挥关键作用，但调控外泌体的机制仍不清楚。
采用高通量测序技术对 6 个样本（3 个猪卵巢颗粒细胞-外泌体共培养样本（GCE）和 3 个猪卵巢颗粒细胞（POGC）样本）的完整转录表达谱进行评估。差异表达分析显示 GCE 组有 924 个 lncRNA、35 个 circRNA、49 个 miRNA 和 9823 个 mRNA。基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析表明，差异表达转录物在与细胞增殖和凋亡相关的途径中富集。此外，基于基因间共表达相关性构建了包含 43 个 lncRNA、6 个 circRNA、11 个 miRNA 和 126 个 mRNA 的 ceRNA 调控网络。该网络中鉴定出 7 个与细胞增殖和凋亡调控相关的 miRNA，包含 92 个亚网络对作为进一步探索外泌体调控机制的候选基因。此外，在细胞水平进行的初步验证表明，外泌体 miR-200b 增强了 POGC 的活力。
转录组分析揭示了一个关键的候选 ceRNA 网络，可能参与外泌体介导的颗粒细胞增殖和凋亡调控，从而影响猪卵泡发育。这些发现为卵泡液外泌体调控的分子机制提供了新的见解，包括编码和非编码 RNA 两个方面。
