Departamento de Genética e Instituto Universitario de Biotecnología y Biomedicina (BIOTECMED), Universidad de Valencia, 46100 Burjassot, Valencia, Spain.
Departamento de Ingeniería Agronómica, Universidad Politécnica de Cartagena, 30203 Cartagena, Spain.
J Invertebr Pathol. 2024 Sep;206:108175. doi: 10.1016/j.jip.2024.108175. Epub 2024 Aug 14.
Orius laevigatus (Hemiptera, Anthocoridae) is a generalist predator extensively used for the biocontrol of diverse agricultural pests. Previous studies on O. laevigatus have focused on the improvement of insect genetic traits, but little is known about its association with microbes, especially viruses that may influence its production and efficacy. More than 280 RNA viruses have been described in other Hemiptera insects, in line with the continuous discovery of insect-specific viruses (ISVs) boosted by next-generation sequencing. In this study, we characterized the repertoire of RNA viruses associated with O. laevigatus. Its virome comprises 27 RNA viruses, classified within fourteen viral families, of which twenty-three viruses are specific to O. laevigatus and four are likely associated with fungal microbiota. The analysis of viral abundance in five O. laevigatus populations confirmed the presence of simultaneous viral infections and highlighted the ubiquitous presence and high abundance of one solinvivirus and three totiviruses. Moreover, we identified 24 non-retroviral endogenous viral elements (nrEVEs) in the genome of O. laevigatus, suggesting a long-term relationship between the host and its virome. Although no symptoms were described in the insect populations under study, the high diversity of viral species and the high abundance of certain RNA viruses identified indicate that RNA viruses may be significant for the applicability and efficacy of O. laevigatus in biocontrol programs.
黄脊长足虻（半翅目，猎蝽科）是一种广泛应用于生物防治多种农业害虫的通用捕食者。先前对黄脊长足虻的研究集中在改善昆虫的遗传特性上，但对其与微生物的关系，尤其是可能影响其繁殖和功效的病毒知之甚少。在其他半翅目昆虫中已经描述了超过 280 种 RNA 病毒，这与下一代测序技术推动的昆虫特异性病毒（ISVs）的不断发现是一致的。在这项研究中，我们描述了与黄脊长足虻相关的 RNA 病毒谱。它的病毒组包括 27 种 RNA 病毒，分为 14 个病毒科，其中 23 种病毒是黄脊长足虻特有的，4 种可能与真菌微生物群有关。对五个黄脊长足虻种群中病毒丰度的分析证实了同时存在病毒感染的情况，并强调了一种索林病毒和三种 totiviruses 的普遍存在和高丰度。此外，我们在黄脊长足虻的基因组中鉴定出 24 种非逆转录病毒内源性病毒元件（nrEVEs），表明宿主与其病毒组之间存在长期的关系。尽管在所研究的昆虫种群中没有描述任何症状，但病毒种类的高度多样性和某些 RNA 病毒的高丰度表明，RNA 病毒可能对黄脊长足虻在生物防治计划中的适用性和功效具有重要意义。
