College of Food Science and Technology, Hebei Agricultural University, Baoding 071000, China.
School of Life Science, Shanxi University, Taiyuan 030006, China.
Int J Biol Macromol. 2024 Oct;278(Pt 2):134758. doi: 10.1016/j.ijbiomac.2024.134758. Epub 2024 Aug 14.
Steam explosion (SE) is a potential method to modify pectin structure, which might be connected to its emulsifying characteristics and the bioavailability of encapsulated polymethoxyflavone like nobiletin. However, the relationship between SE-modified pectin and the bioavailability of encapsulated nobiletin is still unclear. In this study, nobiletin-loaded emulsion was fabricated using citrus pectin modified with SE (0.15-0.9 MPa, 3 min) as emulsifier for in vitro digestion study, and the transport and absorption of nobiletin in Caco-2 cells to investigate the bioavailability-promoting effect. The results showed that SE treatment lowered the droplet size of emulsion from 21.38 ± 2.30 μm to 2.14 ± 0.12 μm, enhanced the nobiletin encapsulation efficiency from 23.73 ± 0.78% to 86.27 ± 3.81%, improved the nobiletin bioaccessibility in vitro from 2.48 ± 0.10% to 25.42 ± 0.10% and increased the intracellular accumulation of nobiletin by over 10 times, even higher than that of Tween 80. In conclusion, pectin from SE-treated citrus peel exhibited good emulsion properties and bioavailability-promoting effect in vitro of nobiletin.
蒸汽爆破（SE）是一种潜在的方法来修饰果胶结构，这可能与其乳化特性以及封装多甲氧基黄酮如诺必灵的生物利用度有关。然而，SE 改性果胶与封装诺必灵的生物利用度之间的关系尚不清楚。在这项研究中，使用 SE 改性的（0.15-0.9 MPa，3 分钟）柑橘果胶作为乳化剂制备了诺必灵负载乳液，用于体外消化研究，并研究了 Caco-2 细胞中诺必灵的转运和吸收，以探讨其促进生物利用度的作用。结果表明，SE 处理将乳液的液滴尺寸从 21.38 ± 2.30 μm降低到 2.14 ± 0.12 μm，将诺必灵包封效率从 23.73 ± 0.78%提高到 86.27 ± 3.81%，体外诺必灵的生物可及性从 2.48 ± 0.10%提高到 25.42 ± 0.10%，并使诺必灵在细胞内的积累增加了 10 倍以上，甚至高于吐温 80。总之，来自 SE 处理的柑橘皮的果胶在体外显示出良好的乳液性能和促进诺必灵生物利用度的作用。
