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果蝇眼中微管系统的空间排列、极性和翻译后修饰。

Spatial arrangement, polarity, and posttranslational modifications of the microtubule system in the Drosophila eye.

机构信息

Unit of Animal Physiology, Institute of Biochemistry and Biology, University of Potsdam, 14476, Potsdam, Germany.

出版信息

Cell Tissue Res. 2024 Nov;398(2):123-137. doi: 10.1007/s00441-024-03914-6. Epub 2024 Aug 17.

DOI:10.1007/s00441-024-03914-6
PMID:39152365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11525301/
Abstract

We have analyzed the organization of the microtubule system in photoreceptor cells and pigment cells within the adult Drosophila compound eye. Immunofluorescence localization of tubulin and of Short stop, a spectraplakin that has been reported to be involved in the anchorage of microtubule minus ends at the membrane, suggests the presence of non-centrosomal microtubule-organizing centers at the distal tip of the visual cells. Ultrastructural analyses confirm that microtubules emanate from membrane-associated plaques at the site of contact with cone cells and that all microtubules are aligned in distal-proximal direction within the photoreceptor cells. Determination of microtubule polarities demonstrated that about 95% of the microtubules in photoreceptor cells are oriented with their plus end in the direction of the synapse. Pigment cells in the eye contain only microtubules aligned in distal-proximal direction, with their plus end pointing towards the retinal floor. There, two populations of microtubules can be distinguished, single microtubules and bundled microtubules, the latter associated with actin filaments. Whereas microtubules in both photoreceptor cells and pigment cells are acetylated and mono/bi-glutamylated on α-tubulin, bundled microtubules in pigment cells are apparently also mono/bi-glutamylated on β-tubulin, providing the possibility of binding different microtubule-associated proteins.

摘要

我们分析了成年果蝇复眼中感光细胞和色素细胞的微管系统的组织。微管蛋白和 Short stop 的免疫荧光定位，Short stop 是一种已经报道参与微管负端在膜上锚定的 spectrin 斑蛋白，提示在视觉细胞的远端末端存在非中心体微管组织中心。超微结构分析证实，微管从与圆锥细胞接触处的膜相关斑块上发出，并且所有微管在感光细胞内沿远端-近端方向排列。微管极性的测定表明，感光细胞中的约 95%的微管以其正极朝向突触的方向定向。眼内的色素细胞仅含有沿远端-近端方向排列的微管，其正极指向视网膜底部。在那里，可以区分两种微管群体，单微管和束状微管，后者与肌动蛋白丝相关联。尽管感光细胞和色素细胞中的微管都在 α-微管上乙酰化和单/双谷氨酸化，并且在色素细胞中的束状微管显然也在 β-微管上单/双谷氨酸化，这提供了结合不同微管相关蛋白的可能性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/78940462ceb8/441_2024_3914_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/63bc1b2c0433/441_2024_3914_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/01e78881eae4/441_2024_3914_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/e13cdc1a8bf4/441_2024_3914_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/d8b30d7864e6/441_2024_3914_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/4f385efc51fa/441_2024_3914_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/bbaa1ca73cd7/441_2024_3914_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/e96adfc1693f/441_2024_3914_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/78940462ceb8/441_2024_3914_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/63bc1b2c0433/441_2024_3914_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/01e78881eae4/441_2024_3914_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/e13cdc1a8bf4/441_2024_3914_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/d8b30d7864e6/441_2024_3914_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/4f385efc51fa/441_2024_3914_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/bbaa1ca73cd7/441_2024_3914_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/e96adfc1693f/441_2024_3914_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ce4/11525301/78940462ceb8/441_2024_3914_Fig8_HTML.jpg

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