College of Food Science and Technology, Shanghai Ocean University, Shanghai, China.
J Sci Food Agric. 2025 Jan 15;105(1):162-170. doi: 10.1002/jsfa.13814. Epub 2024 Aug 16.
Antarctic krill peptide (AKP) has gained considerable interest because of its multiple biological functions. However, its application may be limited by its poor stability and susceptibility to degradation. Encapsulation of AKP using a nanoparticle delivery system is an effective way to overcome these problems. In the present study, bovine serum albumin (BSA) and chitosan (CS) were used as delivery vehicles to encapsulate AKP.
The results revealed that the particle size (83.3 ± 4.4-222.4 ± 32.7 nm) and zeta-potential (35.1 ± 0.7-45.0 ± 2.7 mV) of nanoparticles (NPs) increased with the increasing content of BSA, but the polydispersity index decreased (1.000 ± 0.002 to 0.306 ± 0.011). Hydrogen bonding, hydrophobic and electrostatic interactions were the main forces to form BSA/CS-AKP NPs. X-ray diffraction revealed that AKP was encapsulated by BSA/CS. Scanning electron microscopy images exhibited that the NPs were spherical in shape, uniform in size and tightly bound. BSA/CS-AKP NPs exhibited excellent stability in the pH range (2-5) and after 15 days of storage, and could hinder the release of AKP in simulated gastric environment and promote the release of AKP in simulated intestinal environment. After simulated digestion, the hypoglycemic activity of encapsulated AKP was better than that of unencapsulated AKP.
Our results revealed that the BSA/CS showed great potential for protecting and delivering AKP. © 2024 Society of Chemical Industry.
南极磷虾肽（AKP）具有多种生物学功能，因此受到了广泛关注。然而，其应用可能受到稳定性差和易降解的限制。使用纳米颗粒递送系统将 AKP 包封是克服这些问题的有效方法。本研究使用牛血清白蛋白（BSA）和壳聚糖（CS）作为递送载体来包封 AKP。
结果表明，纳米颗粒（NPs）的粒径（83.3±4.4-222.4±32.7nm）和 Zeta 电位（35.1±0.7-45.0±2.7mV）随 BSA 含量的增加而增加，但多分散指数降低（1.000±0.002 至 0.306±0.011）。氢键、疏水性和静电相互作用是形成 BSA/CS-AKP NPs 的主要作用力。X 射线衍射表明 AKP 被 BSA/CS 包封。扫描电子显微镜图像显示 NPs 呈球形，大小均匀，结合紧密。BSA/CS-AKP NPs 在 pH 值范围（2-5）内和储存 15 天后表现出优异的稳定性，并且可以阻止 AKP 在模拟胃环境中的释放并促进 AKP 在模拟肠环境中的释放。模拟消化后，包封 AKP 的降血糖活性优于未包封 AKP。
我们的结果表明 BSA/CS 具有保护和递送 AKP 的巨大潜力。© 2024 化学工业协会。
