Department of Chemistry, Sardar Vallabhbhai National Institute of Technology, Surat, India.
Research Institute of Chem-Bio Diagnostic Technology, Chung-Ang University, Seoul, Republic of Korea.
Luminescence. 2024 Aug;39(8):e4866. doi: 10.1002/bio.4866.
Copper nanoclusters (Cu NCs) have shown significant attention in sensing of molecular and ionic species. In this work, a single-step biosynthetic approach was introduced for the preparation of fluorescent Cu NCs using Holarrhena pubescens (H. pubescens) leaves extract as a template. The synthesized H. pubescens-Cu NCs act as a nanomolecular probe for the detection of bilirubin in biofluids. The synthesized H. pubescens-Cu NCs displayed highest fluorescence intensity at 454 nm, when excited at 330 nm. Importantly, selective detection of bilirubin was obtained by introducing H. pubescens-Cu NCs as a simple molecular probe. The interaction of bilirubin and H. pubescens-Cu NCs resulted in a remarkable decrease in the emission peak intensity. The developed H. pubescens-Cu NCs-based bilirubin molecular probe has a wide linear range of 0.5-20.00 μM with the limit of detection of 30.54 nM for bilirubin. The promising application of H. pubescens-Cu NCs-based molecular probe was assessed by assaying bilirubin in spiked biofluids.
铜纳米簇 (Cu NCs) 在分子和离子物种的传感中引起了广泛关注。在这项工作中，介绍了一种使用 Holarrhena pubescens (H. pubescens) 叶提取物作为模板的一步法生物合成方法来制备荧光 Cu NCs。合成的 H. pubescens-Cu NCs 可用作生物流体中胆红素的纳米分子探针。当在 330nm 处激发时，合成的 H. pubescens-Cu NCs 在 454nm 处显示出最高的荧光强度。重要的是，通过引入 H. pubescens-Cu NCs 作为简单的分子探针，可以实现对胆红素的选择性检测。胆红素与 H. pubescens-Cu NCs 的相互作用导致发射峰强度显著降低。基于开发的 H. pubescens-Cu NCs 的胆红素分子探针具有 0.5-20.00μM 的宽线性范围，胆红素的检测限为 30.54nM。通过在加标生物流体中测定胆红素来评估基于 H. pubescens-Cu NCs 的分子探针的有前途的应用。
