百草枯毒性及过氧化氢对嗜热细菌的影响。

Paraquat toxicity and effect of hydrogen peroxide on thermophilic bacteria.

作者信息

Allgood G S, Perry J J

出版信息

J Free Radic Biol Med. 1985;1(3):233-7. doi: 10.1016/0748-5514(85)90123-0.

Abstract

Paraquat (PQ++) increased cyanide-resistant univalent respiration in cell suspensions of five strains of obligately thermophilic bacteria. PQ++ was reduced by an NADH: or NADPH:paraquat diaphorase and selectivity for NADH, NADPH, or both electron donors varied among the thermophiles. Superoxide anion production that was dependent on the presence of PQ++ was shown by following the superoxide dismutase-inhibitable reduction of cytochrome c. In addition, the PQ++-dependent formation of hydrogen peroxide from superoxide anion was evident in two of the thermophilic strains. Catalase synthesis was induced by adding hydrogen peroxide to the growth medium of the thermophiles. The induction of catalase to eliminate hydrogen peroxide appears to be an important response of these thermophilic bacteria to oxygen toxicity.

摘要

百草枯（PQ++）增加了五株嗜热厌氧菌细胞悬液中对氰化物有抗性的单价呼吸作用。PQ++可被NADH：或NADPH：百草枯双加氧酶还原，并且嗜热菌对NADH、NADPH或两者电子供体的选择性各不相同。通过跟踪超氧化物歧化酶可抑制的细胞色素c还原，表明超氧阴离子的产生依赖于PQ++的存在。此外，在两株嗜热菌中，超氧阴离子明显依赖PQ++形成过氧化氢。通过向嗜热菌的生长培养基中添加过氧化氢来诱导过氧化氢酶的合成。诱导过氧化氢酶以消除过氧化氢似乎是这些嗜热菌对氧毒性的重要反应。