Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies in Lviv, 50 Pekarska St., Lviv 79010, Ukraine.
Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies in Lviv, 50 Pekarska St., Lviv 79010, Ukraine.
Reprod Biol. 2024 Sep;24(3):100932. doi: 10.1016/j.repbio.2024.100932. Epub 2024 Aug 18.
The effects of Mn-, Zn- or Cu-nanosuccinate added to freezing extender on select post-thaw semen characteristics were determined in six Texel rams (aged 2-4 years) during seasonal anestrus (April-May). Ejaculates (n = 6 per ram) collected into an artificial vagina were divided into ten isovolumetric fractions each. Semen was diluted in lactose-yolk-tris-citrate-glycerin medium and nanosuccinates (Mn- and Zn-nanosuccinate: 0.0 (control), 2.5, 5.0 and 7.5 μg/l; Cu-nanosuccinate: 0.0 (control), 1.25, 2.5 and 3.75 μg/l) were added to semen extender. Extended semen was loaded into 0.25-ml straws and frozen in liquid nitrogen. After thawing, sperm motility parameters were determined with computer assisted semen analysis (CASA), and the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) was measured with a spectrophotometric technique. The addition of 5.0 μg/l of Mn- and Zn-nanosuccinate significantly increased the sperm progressive motility and both 2.5 and 5.0 μg/l improved sperm motion kinetics. Further, both nanosuccinates at a dose of 5.0 μg/l significantly decreased SOD activity and stimulated an increase in GPx and CAT activity in semen samples. Alternatively, the addition of Cu-nanosuccinate (highest dose) significantly reduced the progressive motility and velocity of ram spermatozoa, increased the percentage of sperm with acrosomal/head defects and seminal SOD activity, and depressed CAT (highest dose) and GPx (all doses) activity. In summary, the addition of Mn- and Zn-nanosuccinate to semen extender had beneficial effects on sperm motility/motion kinetics and structural integrity, whereas Cu-nanosuccinate generally had debilitating effects on the post-thaw semen characteristics in rams.
在季节性乏情期（四月至五月），用六只特克塞尔公羊（2-4 岁）来确定在冷冻液中添加锰、锌或铜纳米琥珀酸盐对解冻后精液特性的影响。用人工阴道收集的精液（每只公羊 6 个样本）被分为十个等体积的部分。精液用乳糖-卵黄-三羟甲基氨基甲烷-甘油基介质稀释，并向精液中添加纳米琥珀酸盐（锰和锌纳米琥珀酸盐：0.0（对照）、2.5、5.0 和 7.5μg/l；铜纳米琥珀酸盐：0.0（对照）、1.25、2.5 和 3.75μg/l）。扩展后的精液装入 0.25-ml 细管中并在液氮中冷冻。解冻后，使用计算机辅助精液分析（CASA）测定精子运动参数，并使用分光光度技术测定超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）的活性。添加 5.0μg/l 的锰和锌纳米琥珀酸盐可显著提高精子的前向运动能力，2.5 和 5.0μg/l 可改善精子运动动力学。此外，两种纳米琥珀酸盐在 5.0μg/l 的剂量下均可显著降低 SOD 活性，并刺激精液样本中 GPx 和 CAT 活性的增加。相反，添加铜纳米琥珀酸盐（最高剂量）可显著降低公羊精子的前向运动能力和速度，增加顶体/头部缺陷的精子比例，并抑制 CAT（最高剂量）和 GPx（所有剂量）活性。总之，在精液中添加锰和锌纳米琥珀酸盐对精子运动/运动动力学和结构完整性有有益影响，而铜纳米琥珀酸盐通常对公羊解冻后的精液特性有不良影响。
