Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou, China; China Greater Bay Area Research Center of Environmental Health, School of Medicine, Jinan University, Guangzhou, China.
School of Public Health, Guangdong Pharmaceutical University, Guangzhou, China.
Environ Int. 2024 Sep;191:108957. doi: 10.1016/j.envint.2024.108957. Epub 2024 Aug 14.
Endocrine-disrupting chemicals (EDCs) exhibited the detriment in female reproductive health. Our objective was to investigate the individual and mixture effects of EDCs present in follicular fluid, the environment in which oocytes grow and develop, on early reproductive outcomes. We recruited 188 women seeking reproduction examination from the Study of Exposure and Reproductive Health (SEARCH) cohort between December 2020 and November 2021. We assessed the concentrations of 7 categories of 64 EDCs in follicular fluid, and measured early reproductive outcomes, including retrieved oocytes, mature oocytes, normal fertilized oocytes, and high-quality embryos. In this study Monomethyl phthalate (MMP) (2.17 ng/ml) were the compounds found in the highest median concentrations in follicular fluid. After adjusting for multiple testing, multivariate regression showed that multiple EDCs were significantly negatively associated with early assisted reproduction outcomes. For example, MMP showed a significant negative correlation with the number of high quality embryos (β: -0.1, 95 % CI: -0.15, -0.04). Specifically, eight types of EDCs were significantly negatively associated with four early assisted reproductive outcomes (β range: -0.2 ∼ -0.03). In the mixed exposure model, we found that mixtures of EDC were significantly negatively correlated with all four outcomes. In the quantile g-computation (QGCOMP) model, for each interquartile range increase in the concentration of EDC mixtures, the number of oocytes retrieved, mature oocytes, normally fertilized oocytes, and high-quality embryos decreased by 0.46, 0.52, 0.77, and 1.2, respectively. Moreover, we identified that phthalates (PAEs) predominantly contributed to the negative effects. Future research should validate our findings.
环境内分泌干扰物（EDCs）对女性生殖健康有不良影响。我们的目的是研究卵泡液中存在的 EDC 对卵母细胞生长和发育的环境的个体和混合效应，以评估其对早期生殖结局的影响。我们招募了 188 名 2020 年 12 月至 2021 年 11 月期间参加暴露与生殖健康研究（SEARCH）队列的寻求生殖检查的女性。我们评估了卵泡液中 7 类 64 种 EDC 的浓度，并测量了早期生殖结局，包括可回收的卵母细胞、成熟卵母细胞、正常受精的卵母细胞和高质量胚胎。在这项研究中，邻苯二甲酸单甲酯（MMP）（2.17ng/ml）是卵泡液中发现的浓度最高的化合物。在进行多重检验调整后，多元回归显示，多种 EDC 与早期辅助生殖结局显著负相关。例如，MMP 与高质量胚胎数量呈显著负相关（β：-0.1，95%CI：-0.15，-0.04）。具体而言，8 种 EDC 与 4 种早期辅助生殖结局显著负相关（β范围：-0.2~-0.03）。在混合暴露模型中，我们发现 EDC 混合物与所有 4 种结局均显著负相关。在分位数 g 计算（QGCOMP）模型中，EDC 混合物浓度每增加一个四分位间距，可回收卵母细胞、成熟卵母细胞、正常受精的卵母细胞和高质量胚胎的数量分别减少 0.46、0.52、0.77 和 1.2。此外，我们发现邻苯二甲酸酯（PAEs）是主要的负面影响因素。未来的研究应验证我们的发现。
