MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China; The People's Government of Zougang Town, Xiaochang County, Xiaogan City, Hubei, 432910, China.
Plant Physiol Biochem. 2024 Oct;215:109047. doi: 10.1016/j.plaphy.2024.109047. Epub 2024 Aug 14.
The formation of rice aroma is a complex process that is influenced by genetic and environmental factors. More than 500 fragrance compounds have been documented in fragrant rice, among which 2-AP dominates the aroma of rice. This paper introduced the identification of OsBadh2 in the biosynthesis of 2-AP in rice. Then, non-enzymatic and enzymatic pathways of the 2-AP biosynthesis have been comprehensively investigated. In detail, 2-AP biosynthesis-associated enzyme, such as OsBADH2, OsP5CS, OsGAD, OsGAPDH, OsProDH, OsOAT, OsODC and OsDAO, have been summarized, while MG and fatty acids are also implicated in modulating the biosynthesis of 2-AP by providing the acetyl groups. Moreover, extensive collections of traditional fragrant rice varieties have been collated, together with the OsBadh2 haplotypes of 312 fragrant rice germplasm in China. And finally, genetic engineering of OsBadh2 and other genes in the 2-AP biosynthesis to develop fragrant rice are discussed.
稻米香气的形成是一个复杂的过程，受到遗传和环境因素的影响。在香米中已经鉴定出超过 500 种香气化合物，其中 2-AP 主导着稻米的香气。本文介绍了 OsBadh2 在水稻 2-AP 生物合成中的鉴定。然后，全面研究了 2-AP 生物合成的非酶和酶途径。详细地，总结了与 2-AP 生物合成相关的酶，如 OsBADH2、OsP5CS、OsGAD、OsGAPDH、OsProDH、OsOAT、OsODC 和 OsDAO，而 MG 和脂肪酸也通过提供乙酰基来参与调节 2-AP 的生物合成。此外，还整理了大量的传统香米品种，并对中国 312 份香稻种质资源的 OsBadh2 单倍型进行了分析。最后，讨论了通过遗传工程改造 OsBadh2 和 2-AP 生物合成中的其他基因来开发香稻的问题。
