Department of Veterinary Medicine, University of Teramo, Teramo, Italy.
Vet Practitioner, Teramo, Italy.
Ann Parasitol. 2024;70(2):113-118. doi: 10.17420/ap7002.528.
Duddingtonia flagrans is a nematode trapping fungus used for the control of gastrointestinal nematodes in livestock. The quantity of chlamydospores of D. flagrans required for the reduction of third-stage larvae (L3) of sheep gastrointestinal nematodes (GIN) is largely unknown, and a matter of discussion. The aim of this experiment was to determine in vitro the nematophagous activity of four different concentrations of D. flagrans (1000, 3000, 6250, or 11000 chlamydospores/ml) in the presence of varying numbers of GIN third-stage larvae (L3) (500, 1000, 1500). Additionally, the study sought to evaluate the efficacy of this fungus on Haemonchus contortus, Teladorsagia circumcincta, Trichostrongylus colubriformis and Chabertia ovina. The results showed that as fungal concentrations increased, so did the larval reduction of third-stage infective larvae in each test. L3s number was not a determining factor in the efficacy against GIN. The comparison between various concentrations of chlamydospores revealed significant differences, particularly between 1000 and 11000 chlamydospores (P≤0.05). Regarding the larval reduction of the GIN species considered, D. flagrans demonstrated the same effectiveness across all species tested. The results of the current study confirm the efficacy and underscore the importance of D. flagrans as an alternative for controlling of GIN.
鲜亮枝孢菌是一种寄生性线虫真菌，用于控制家畜的胃肠道线虫。减少绵羊胃肠道线虫（GIN）第三期幼虫（L3）所需的鲜亮枝孢菌分生孢子数量在很大程度上是未知的，也是一个讨论的话题。本实验的目的是确定在体外不同浓度的鲜亮枝孢菌（1000、3000、6250 或 11000 分生孢子/ml）在存在不同数量的 GIN 第三期幼虫（L3）（500、1000、1500）时的捕食线虫活性。此外，该研究还评估了该真菌对捻转血矛线虫、奥斯特线虫、哥伦比亚食道口线虫和绵羊夏伯特线虫的功效。结果表明，随着真菌浓度的增加，每种试验中第三期感染幼虫的幼虫减少量也随之增加。L3 的数量不是影响 GIN 功效的决定因素。分生孢子的各种浓度之间的比较显示出显著差异，特别是在 1000 与 11000 分生孢子之间（P≤0.05）。关于所考虑的 GIN 物种的幼虫减少，鲜亮枝孢菌在所有测试的物种中表现出相同的效果。本研究的结果证实了其功效，并强调了鲜亮枝孢菌作为控制 GIN 的替代方法的重要性。
