波瑞黄酮B通过抑制炎症和JAK2/STAT3信号通路对糖尿病肾病大鼠的肾脏保护作用
Renal Protective Effect of Boeravinone B against Diabetic Nephropathy Rats via Inhibition of The Inflammatory and JAK2/STAT3 Signalling Pathway.
作者信息
Wen Wenbin, Sun Jian, Ma Yanmei, Shi Shuaishuai, Zhang Wei, Li Junyan, Guo Huidan
机构信息
Department of Nephrology, Heji Hospital Affiliated to Changzhi Medical College, Changzhi, China.
Blood Purification Center, Qingdao Municipal Hospital, Qingdao, China.
出版信息
Cell J. 2024 Aug 11;26(6):351-360. doi: 10.22074/cellj.2024.2017978.1468.
OBJECTIVE
Chronic inflammation is a common feature in diabetes, especially when blood sugar levels are poorly controlled. This chronic low-grade inflammation can affect various organs, including the kidneys. Podocyte damage play a key role in the development of diabetic nephropathy (DN). The aim of the study was to evaluate the nephroprotective effect of Boeravinone B (BB) against streptozotocin (STZ) induced DN in rats and explore the underlying mechanism.
MATERIALS AND METHODS
In this experimental study, the rats received intraperitoneal injections of STZ (60 mg/kg) to induce DN. Various doses of BB (2.5, 5, and 7.5 mg/kg) were administered orally. Glucose levels, body weights, and organ weights (hepatic and renal) were assessed. Renal, histomorphological, antioxidant, hepatic, and cytokine levels were determined, as were the mRNA expression levels of and . At end of the experimental study, the rats were sacrificed and their renal tissues were removed for histopathological assessment.
RESULTS
BB treatment decreased glucose levels and increased body weights. This treatment suppressed hepatic weights, increased renal tissue weights, and also decreased renal parameters like uric acid, urea, bilirubin, creatinine (Cr) and, albumin. There was a decrease (P<0.001) in histomorphological parameters such as kidney hypertrophy index (KHI), mean glomerular volume (MGV), foot process fusion ratio (FPFR), and glomerular basement membrane thickness (GBMT) after treatment with BB. In addition, this treatment improved the levels of renal podocin, renal CD2- associated protein (CD2AP) and suppressed hepatic parameter levels. BB treatment (P<0.001) altered antioxidant parameters and cytokine levels, and suppressed mRNA expressions of and .
CONCLUSION
Administration of BB showed renal protective effects against STZ-induced DN in rats via the reduction of oxidative stress and inflammatory reactions.
目的
慢性炎症是糖尿病的常见特征，尤其是血糖水平控制不佳时。这种慢性低度炎症会影响包括肾脏在内的多个器官。足细胞损伤在糖尿病肾病（DN）的发展中起关键作用。本研究旨在评估波叶山柑酮B（BB）对链脲佐菌素（STZ）诱导的大鼠糖尿病肾病的肾保护作用，并探讨其潜在机制。
材料与方法
在本实验研究中，大鼠腹腔注射STZ（60 mg/kg）诱导糖尿病肾病。口服给予不同剂量的BB（2.5、5和7.5 mg/kg）。评估血糖水平、体重和器官重量（肝脏和肾脏）。测定肾脏、组织形态学、抗氧化、肝脏和细胞因子水平，以及 和 的mRNA表达水平。实验研究结束时，处死大鼠并取出肾脏组织进行组织病理学评估。
结果
BB治疗可降低血糖水平并增加体重。该治疗抑制肝脏重量，增加肾脏组织重量，还降低了尿酸、尿素、胆红素、肌酐（Cr）和白蛋白等肾脏参数。用BB治疗后，肾脏肥大指数（KHI）、平均肾小球体积（MGV）、足突融合率（FPFR）和肾小球基底膜厚度（GBMT）等组织形态学参数降低（P<0.001）。此外，该治疗改善了肾脏足突蛋白、肾脏CD2相关蛋白（CD2AP）水平，并抑制了肝脏参数水平。BB治疗（P<0.001）改变了抗氧化参数和细胞因子水平，并抑制了 和 的mRNA表达。
结论
给予BB可通过减轻氧化应激和炎症反应对STZ诱导的大鼠糖尿病肾病发挥肾脏保护作用。
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