School of Life Sciences and Technology, Bandung Institute of Technology, 10 Ganeca Street, Bandung, West Java 40132, Indonesia.
Department of Biology, Faculty of Sciences and Technology, Syarif Hidayatullah State Islamic University Jakarta, 95 Ir. H. Juanda Street, Cempaka Putih, East Ciputat, South Tangerang, Banten 15412, Indonesia.
Trop Biomed. 2024 Jun 1;41(2):142-148. doi: 10.47665/tb.41.2.002.
Salmonella enterica subsp. enterica serovar Enteritidis (SE) is a global concern for the poultry industry due to its association with foodborne illnesses. The transmission occurs through the transovarial route which initiates from colonization in oviducts and ascending to ovaries. Though there are studies on cytosine-phosphate-guanine oligodeoxynucleotide (CpG-ODN) and the increase of innate immune response, there is limited research on the intravaginal treatment using CpG-ODN. Previous studies have shown that stimulating CpG-ODN can induce the production of antimicrobial peptide avian beta-defensins (AvBDs) in vaginal cell cultures, there is limited information on the use of intravaginal treatment to induce the innate immune system, particularly in the Kampung Unggul Balitbangtan (KUB-1) chickens (Gallus gallus domesticus). This study investigates the impact of intravaginal CpG-ODN stimulation on the innate immune response in KUB-1 chicken ovaries and oviducts when challenged to SE. A total of 39 KUB-1 chickens were divided into four groups namely T1 (treated with CpG-ODN, n=12), T2 (SE group, n=12), T3 (CpG-ODN and SE, n=12), and Control (without CpG-ODN and SE, n=3). Chickens were observed from day 1 to 4 post-intravaginal (PI) inoculation. The results suggest that intravaginal CpG-ODN treatment modulates AvBD10 production through toll-like receptor (TLR)21, with interleukin (IL)1B and IL10 playing reciprocal roles, providing insights into the potential of this treatment to prevent transovarial Salmonellosis in poultry. The novelty of this study adds valuable insights to the current body of knowledge.
肠炎沙门氏菌亚种肠炎血清型（SE）是家禽业关注的全球性问题，因为它与食源性疾病有关。这种传播是通过经卵传播途径发生的，从输卵管定植开始，然后向上传播到卵巢。虽然已经有关于胞嘧啶-磷酸-鸟嘌呤寡脱氧核苷酸（CpG-ODN）和固有免疫反应增加的研究，但关于阴道内使用 CpG-ODN 的治疗方法的研究有限。以前的研究表明，刺激 CpG-ODN 可以诱导阴道细胞培养物中抗菌肽禽β-防御素（AvBDs）的产生，关于阴道内治疗方法来诱导固有免疫系统的信息有限，特别是在 Kampung Unggul Balitbangtan（KUB-1）鸡（Gallus gallus domesticus）中。本研究调查了阴道内 CpG-ODN 刺激对 KUB-1 鸡卵巢和输卵管固有免疫反应的影响，当这些鸡受到 SE 挑战时。总共 39 只 KUB-1 鸡被分为四组，即 T1（用 CpG-ODN 处理，n=12）、T2（SE 组，n=12）、T3（CpG-ODN 和 SE，n=12）和对照（未用 CpG-ODN 和 SE，n=3）。鸡从阴道内（PI）接种后第 1 天到第 4 天进行观察。结果表明，阴道内 CpG-ODN 处理通过 Toll 样受体（TLR）21 调节 AvBD10 的产生，白细胞介素（IL）1B 和 IL10 发挥相互作用，为这种治疗方法预防家禽经卵传播沙门氏菌病提供了新的见解。本研究的新颖性为现有知识体系增添了宝贵的见解。
