College of Veterinary Medicine, College of Animal Medicine, Northeast Agricultural University, Harbin 150030, China.
College of Veterinary Medicine, College of Animal Medicine, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China; Laboratory of Embryo Biotechnology, College of Life Science, Department of Biotechnology, Northeast Agricultural University, Harbin, 150030, China.
Poult Sci. 2024 Nov;103(11):104179. doi: 10.1016/j.psj.2024.104179. Epub 2024 Aug 6.
Naringenin is a flavonoid with significant anti-inflammatory and antioxidant properties. Mitochondrial dynamics, the mitochondrial respiratory chain, and mtROS are closely related to each other and regulate various biological processes. Ferroptosis is closely related to inflammatory responses and immune function in multiple tissues and organs. However, whether naringenin can alleviate LPS-induced inflammation and immune disorders in the chicken thymus via mtROS/ferroptosis has not been reported. Therefore, in this study, we constructed chicken thymus and MSB-1 cell models of LPS and naringenin based on screening for naringenin concentrations that have positive effects on inflammation and immune function to further investigate the anti-inflammatory, antiferroptosis, and maintenance of the immune function of naringenin. The results showed that 40 mg/kg naringenin alleviated LPS-induced tissue damage, elevated serum inflammatory factors, and decreased serum immune factors. The mechanism by which naringenin attenuates mtROS release by alleviating the imbalance of mitochondrial dynamics and the blockage of the respiratory chain. The effect of naringenin on alleviating LPS-induced lipid peroxidation, disruption of the GSH/GSSG system, iron overload, and GPx4 inactivation, thereby attenuating ferroptosis in thymus tissue, was inhibited by the addition of mtROS activators. In conclusion, naringenin alleviates LPS-induced ferroptosis in chicken thymus by attenuating mtROS release.
柚皮素是一种具有显著抗炎和抗氧化特性的类黄酮。线粒体动力学、线粒体呼吸链和 mtROS 密切相关，共同调节着各种生物学过程。铁死亡与多个组织和器官中的炎症反应和免疫功能密切相关。然而，柚皮素是否可以通过 mtROS/铁死亡来减轻 LPS 诱导的鸡胸腺炎症和免疫紊乱尚未有报道。因此，在本研究中，我们构建了 LPS 和柚皮素作用下的鸡胸腺和 MSB-1 细胞模型，通过筛选对炎症和免疫功能具有积极作用的柚皮素浓度，进一步研究了柚皮素的抗炎、抗铁死亡和维持免疫功能的作用。结果表明，40mg/kg 的柚皮素缓解了 LPS 诱导的组织损伤，提高了血清炎症因子水平，降低了血清免疫因子水平。柚皮素通过减轻线粒体动力学失衡和呼吸链阻断来抑制 mtROS 的释放，从而减轻炎症反应。柚皮素对缓解 LPS 诱导的脂质过氧化、GSH/GSSG 系统破坏、铁过载和 GPx4 失活，从而减轻胸腺组织铁死亡的作用，可以被 mtROS 激活剂所抑制。综上所述，柚皮素通过抑制 mtROS 的释放来减轻 LPS 诱导的鸡胸腺铁死亡。
