State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases/Key Laboratory for Zoonosis Research of the Ministry of Education, College of Food Science and Engineering, Jilin University, Changchun 130062, China.
Department of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi Arabia.
Int J Biol Macromol. 2024 Oct;278(Pt 2):134826. doi: 10.1016/j.ijbiomac.2024.134826. Epub 2024 Aug 21.
Forest frog's oviduct oil (FFOO) is highly susceptible to microbial spoilage during storage, which causes serious safety concerns and economic losses. However, little information is available regarding the preservation of it up to now. The aim of this research is to understand the dominant microbial community of FFOO spoilage, and based on this, develop a kind of edible nanoemulsion coating for preserving FFOO. Microbial metagenomic analysis indicated that the Aspergillus genus increased significantly during storage. In the present study, gum arabic and whey protein isolate were chosen as the coating matrix, the natural compounds sanguinarine and glabridin were selected as antimicrobial agents to prepare double-layer nanoemulsion edible coating. When the ratio of sanguinarine and glabridin in the nanoemulsion was 1:3, it exhibited strongest storage stability and antifungal activity. The mycelial inhibition rate of 1:3 nanoemulsion against dominant microbial community (Aspergillus niger and Aspergillus glaucus) reached 88.89 ± 1.37 % and 89.68 ± 1.37 %, respectively. The experimental results indicated that the edible nanoemulsion coating not only had outstanding antifungal activity, but also had excellent fresh-keeping effect on FFOO. This nanoemulsion coating could be a promising and potential candidate for food preservation.
林蛙输卵管油（FFOO）在储存过程中极易受到微生物的破坏，这引起了严重的安全问题和经济损失。然而，到目前为止，关于它的保存方法的信息还很少。本研究旨在了解 FFOO 腐败的主要微生物群落，并在此基础上开发一种可食用的纳米乳液涂层来保存 FFOO。微生物宏基因组分析表明，在储存过程中，曲霉属的数量显著增加。在本研究中，选择阿拉伯胶和乳清蛋白分离物作为涂层基质，选择天然化合物血根碱和甘草素作为抗菌剂来制备双层纳米乳液可食用涂层。当纳米乳液中血根碱和甘草素的比例为 1:3 时，它表现出最强的储存稳定性和抗真菌活性。1:3 纳米乳液对优势微生物群落（黑曲霉和青霉）的抑菌率分别达到 88.89±1.37%和 89.68±1.37%。实验结果表明，这种可食用的纳米乳液涂层不仅具有出色的抗真菌活性，而且对 FFOO 具有极好的保鲜效果。这种纳米乳液涂层可能是一种有前途和潜在的食品保鲜候选物。
