Chen Nan, Song Lin, Wang Xinqiao, Liu Herong, Fan Shengnan, Song Hui
School of Public Health, Ningxia Medical University, Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Yinchuan 750004, China.
the Second Affiliated Hospital of Ningxia Medical University, the First People's Hospital of Yinchuan, Yinchuan 750004, China.
Wei Sheng Yan Jiu. 2024 Jul;53(4):561-568. doi: 10.19813/j.cnki.weishengyanjiu.2024.04.007.
To explore the association of Occupational chronic psychological stress with transaminase, heat shock protein70(HSP70)gene family and their protein interaction with metabolic syndrome(MS).
A case-control study was used. According to the inclusion and exclusion criteria, from March 2015 to March 2016, 583 unrelated MS patients were selected as the case group and 585 unrelated healthy people as the control group among hospitalized and physical examination subjects aged 20-60 in Wuzhong People's Hospital and General Hospital of Ningxia Medical University. Questionnaire survey, physical examination, clinical and biochemical indicators, serum HSP70 level and five-locus polymorphism detection of HSP70 gene were carried out. GMDR 0.7 software was used to analyze the relationship between psychological stress, transaminase, HSP70 gene and its protein interaction and MS.
After adjusting for age and sex, the rs1008438, rs1061581, rs539689 and rs222795 locus of HSP70 gene in the Co-dominant model and Dominant model and the rs222795 loci in the Over-dominant model carry wild homozygous genotype and heterozygous genotype were all related to the reduction of MS risk(OR<1, P<0.05). GMDR result: the 2-factor interaction model composed of psychological stress and serum HSP70, the 2-3 factor interaction model composed of transaminase activity, and the 2-6 factor interaction model composed of five locus of HSP70 gene, the 2-9 factor interaction model consisting of psychological stress and transaminase activity, HSP70 gene and its protein were all significantly associated with MS(P<0.01, P<0.05), all each factor interaction models were the best, and the 9-factor optimal interaction model had the highest risk of MS(OR=46.51, 95%CI 27.65-78.26), and the risk of MS in high-risk type was 45.23 times higher than that in low-risk type(95%CI 31.29-65.38, P<0.01).
HSP70 gene family carrying wild-type alleles is a protective factor for MS. The interaction among Occupational chronic psychological stress interacts with transaminases, HSP70 gene and its serum proteins may be associated with MS. With the increase of involvement interaction factors, the risk of MS increased significantly. The interaction of multiple factors can greatly increase its risk.
探讨职业性慢性心理应激与转氨酶、热休克蛋白70（HSP70）基因家族及其蛋白相互作用与代谢综合征（MS）的关联。
采用病例对照研究。根据纳入和排除标准，2015年3月至2016年3月，在吴忠市人民医院和宁夏医科大学总医院20 - 60岁的住院及体检人群中，选取583例无亲缘关系的MS患者作为病例组，585例无亲缘关系的健康人作为对照组。进行问卷调查、体格检查、临床及生化指标检测、血清HSP70水平检测及HSP70基因五位点多态性检测。应用GMDR 0.7软件分析心理应激、转氨酶、HSP70基因及其蛋白相互作用与MS的关系。
校正年龄和性别后，HSP70基因的rs1008438、rs1061581、rs539689和rs222795位点在共显性模型和显性模型中以及rs222795位点在超显性模型中携带野生纯合基因型和杂合基因型均与MS风险降低相关（OR<1，P<0.05）。GMDR结果显示：心理应激与血清HSP70组成的双因素交互作用模型、转氨酶活性组成的2 - 3因素交互作用模型、HSP70基因五位点组成的2 - 6因素交互作用模型、心理应激与转氨酶活性、HSP70基因及其蛋白组成的2 - 9因素交互作用模型均与MS显著相关（P<0.01，P<0.05），各因素交互作用模型均为最佳，9因素最优交互作用模型的MS风险最高（OR = 46.51，95%CI 27.65 - 78.26），高危型MS风险是低危型的45.23倍（95%CI 31.29 - 65.38，P<0.01）。
携带野生型等位基因的HSP70基因家族是MS的保护因素。职业性慢性心理应激与转氨酶、HSP70基因及其血清蛋白之间的相互作用可能与MS相关。随着参与交互作用因素的增加，MS风险显著增加。多因素的相互作用可大幅增加其风险。
