College of Food Science and Technology, Bohai University, Jinzhou, Liaoning, China.
Collaborative Innovation Center for Genetic Improvement and High Quality and Efficiency Production of Northeast Japonica Rice in China, Shenyang Agricultural University, Shenyang, Liaoning, China.
Int J Biol Macromol. 2024 Oct;278(Pt 4):134849. doi: 10.1016/j.ijbiomac.2024.134849. Epub 2024 Aug 17.
Genes play a pivotal role in regulating the germination of cereal grains; however, there is limited research on the impact of germination genes on the physicochemical properties of germinated cereal starch. We investigated the effects of the OsGA20ox1 gene on the multiscale structural features and adhesion behavior of germinated brown rice starch. Compared to the knockout lines group, the wild type exhibited a decrease in double-helix content (62.74 %), relative crystallinity (47.39 %), and short-range molecular ordering (2.47 %), accompanied by enhanced erosion on the surface of starch granules. The damage to glycosidic bonds at the double-helix level and the heightened structural amorphization (90.95 %) led to reduced entanglement and interaction among starch molecules, ultimately resulting in reduced characteristic viscosity. Further transcriptomic analysis revealed that OsGA20ox1 could regulate the expression of starch-related enzyme genes in the starch metabolism pathway during germination of brown rice. This study contributes to understanding the role of germination genes in promoting the physicochemical properties of starch in germinated grains, thereby opening up new avenues for the improvement of plant-based starch, and paving the way for further research in this field.
基因在调控谷物种子萌发中起着关键作用；然而，关于萌发基因对萌发谷物淀粉理化性质影响的研究还很有限。我们研究了 OsGA20ox1 基因对发芽糙米淀粉的多尺度结构特征和黏附行为的影响。与敲除系相比，野生型的双螺旋含量（62.74%）、相对结晶度（47.39%）和短程分子有序性（2.47%）降低，同时淀粉颗粒表面的侵蚀增强。双螺旋水平糖苷键的破坏和结构非晶化程度的增加（90.95%）导致淀粉分子之间缠结和相互作用减少，最终特征粘度降低。进一步的转录组分析表明，OsGA20ox1 可以在糙米萌发过程中调节淀粉代谢途径中与淀粉相关的酶基因的表达。本研究有助于理解萌发基因在促进萌发谷物中淀粉理化性质方面的作用，为植物淀粉的改良开辟了新的途径，并为该领域的进一步研究奠定了基础。
