Shandong Provincial Key Laboratory of Detection Technology for Tumor Markers, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276000, China.
College of Marine Sciences, Beibu Gulf University, Qinzhou 535011, China.
Anal Chem. 2024 Sep 3;96(35):14215-14221. doi: 10.1021/acs.analchem.4c02881. Epub 2024 Aug 20.
Atherosclerosis (AS) is the leading cause of cardiovascular disease. Foam cells, with elevated lipid droplets (LDs) and HClO levels, are the main components of the atherosclerotic plaques that are characteristic of AS. Super-resolution imaging can be used to visualize the distribution of LDs in foam cells at the nanometer level, facilitating the identification of LDs and HClO. In the present study, we report the development of a ratiometric fluorescent probe, , for super-resolution imaging of LDs and HClO. Super-resolution imaging with this probe revealed the precise structure of LDs at the suborganelle level. Moreover, the fluorescence behavior of on the surface of LDs verified its excellent performance in detecting HClO in the foam cells. can sequentially and specifically respond to LDs and HClO "turn-on" and ratiometric signal output, respectively, thus contributing to precise imaging of foam cells. Importantly, we demonstrate that can be used to report on upregulated HClO in atherosclerotic plaques in the aorta of AS mice, providing a suitable fluorescent tool for early atherosclerotic disease assessment.
动脉粥样硬化（AS）是心血管疾病的主要原因。泡沫细胞是 AS 特征性粥样斑块的主要组成部分，其富含脂质滴（LDs）和次氯酸（HClO）。超分辨率成像可用于在纳米级水平上可视化泡沫细胞中 LDs 的分布，从而有助于识别 LDs 和 HClO。在本研究中，我们报告了一种比率型荧光探针的开发，用于 LDs 和 HClO 的超分辨率成像。使用该探针进行超分辨率成像揭示了 LDs 在亚细胞器水平的精确结构。此外，探针在 LDs 表面的荧光行为验证了其在检测泡沫细胞中 HClO 方面的优异性能。探针可以分别、特异性地对 LDs 和 HClO 进行“开启”和比率信号输出响应，从而有助于对泡沫细胞进行精确成像。重要的是，我们证明可以用它来报告 AS 小鼠主动脉粥样硬化斑块中 HClO 的上调，为早期动脉粥样硬化疾病评估提供了一种合适的荧光工具。
