Rudchenko O N
Mol Gen Mikrobiol Virusol. 1985 May(5):36-41.
The effect of resident plasmid pLD4, a derivative of plasmid Hly241, on transformability of the host bacteria cells has been studied. Plasmid pLD4 was transferred into the different strains of E. coli subsequently transformed by the DNA of plasmids pBR322, pBR325, pAL-R2, pMB9. The majority of strains harbouring pLD4 obtain the increased ability to be transformed as compared with the ability of isogenic plasmidless strains. The similar but less expressed effect was conferred by the plasmid Hly241. Another hemolytic plasmid Hly195 and its derivatives, carrying the different transposons, as well as plasmid F' tet Hly did not increase the transformability of host bacteria. The optimal parameters for transformation of the strains harbouring pLD4 and plasmidless strains coincide, but the number of competent cells is considerably higher for plasmid containing strains, due to preliminary results.
研究了质粒Hly241的衍生物——常驻质粒pLD4对宿主细菌细胞转化能力的影响。随后将质粒pLD4转入不同的大肠杆菌菌株，这些菌株再用质粒pBR322、pBR325、pAL-R2、pMB9的DNA进行转化。与同基因无质粒菌株相比，大多数携带pLD4的菌株获得了增强的转化能力。质粒Hly241也有类似但较弱的效果。另一种携带不同转座子的溶血性质粒Hly195及其衍生物，以及质粒F' tet Hly并未提高宿主细菌的转化能力。携带pLD4的菌株和无质粒菌株的最佳转化参数一致，但根据初步结果，含质粒菌株的感受态细胞数量要高得多。
