Kurits T S, Selivanov N A, Mesianzhinov V V
Mol Gen Mikrobiol Virusol. 1985 Aug(8):29-31.
Short-tail fibers (STF) of bacteriophage T4 are a polyfunctional protein. STF appears to be a trimer of gene 12 product. The modified trimers, consisting of fragments of gene 12 product with mol mass 45 and 50 Kd, respectively, were isolated by limited proteolysis with trypsin and papain. The isolated trimers retained their bactericidal activity but were unable to complement the fiberless phage particles. The results obtained suggest that STF loci responsible for bactericidal effect are separated from the loci involved in interaction with the base plate.
噬菌体T4的短尾纤维（STF）是一种多功能蛋白质。STF似乎是基因12产物的三聚体。通过用胰蛋白酶和木瓜蛋白酶进行有限的蛋白水解，分离出由分子量分别为45和50 Kd的基因12产物片段组成的修饰三聚体。分离出的三聚体保留了它们的杀菌活性，但无法补充无纤维的噬菌体颗粒。所获得的结果表明，负责杀菌作用的STF位点与参与与基板相互作用的位点是分开的。
