Weintraub D, Masek M, Billingham M E
J Heart Transplant. 1985 Feb;4(2):213-6.
Monoclonal antibodies are being used as specific therapeutic agents for treating organ rejection. This type of treatment requires that the pattern and type of infiltrating cells in the graft during acute rejection be defined. In this study 24 endomyocardial biopsies from cyclosporine-treated human heart allograft recipients were obtained during acute rejection. The biopsies were stained with immunological markers to define the infiltrating cells. The biopsies were divided into three groups; mild, moderate and resolving acute rejection. In all groups T-lymphocytes (marked with the monoclonal Leu-4) were the predominant cells with absence of B-lymphocytes (marked with T015). The results of this study showed that cyclosporine-treated heart transplant recipients have a mixed suppressor/cytotoxic (Leu-2a) and helper/inducer (Leu-3) myocardial infiltrate in contrast to the biopsies of recipients treated with azathioprine, in which suppressor/cytotoxic T-lymphocytes (Leu-2a) were predominant. A clear sequential pattern of changes in the T-cell subpopulations did not emerge, although macrophages became more prevalent with resolving rejection.
单克隆抗体正被用作治疗器官排斥反应的特异性治疗药物。这类治疗需要明确急性排斥反应期间移植物中浸润细胞的模式和类型。在本研究中，从接受环孢素治疗的人类心脏移植受者身上获取了24份急性排斥反应期间的心肌内膜活检样本。活检样本用免疫标记物染色以确定浸润细胞。活检样本分为三组：轻度、中度和消退期急性排斥反应。在所有组中，T淋巴细胞（用单克隆抗体Leu-4标记）是主要细胞，而B淋巴细胞（用T015标记）不存在。本研究结果表明，与接受硫唑嘌呤治疗的受者的活检样本不同，接受环孢素治疗的心脏移植受者有混合的抑制性/细胞毒性（Leu-2a）和辅助性/诱导性（Leu-3）心肌浸润，在接受硫唑嘌呤治疗的受者的活检样本中，抑制性/细胞毒性T淋巴细胞（Leu-2a）占主导。虽然随着排斥反应的消退巨噬细胞变得更为普遍，但并未出现T细胞亚群变化的清晰连续模式。
