Miller L W, Jellinek M, Codd J E, Kolata R J
J Heart Transplant. 1985 May;4(3):319-24.
Simple cold storage remains the current method of preservation of human heart allografts between removal and transplantation, but this technique is not adequate for prolonged periods of preservation. An alternative to cold storage is machine perfusion of the isolated organ. Although this technique has yielded promising results in kidney preservation, there is limited experience with the heart. One factor limiting the effectiveness of perfusion preservation is the toxicity of oxygen free radicals generated during the perfusion. The generation and reduction of these radicals results in an electron transfer, producing an electrochemical potential known as the redox potential. This study examines the role of machine perfusion in heart preservation with and without the use of a new electrochemical cell that is able to control the redox potential during the perfusion. Hearts were preserved for twenty-four hours by either simple cold storage (Group I), or by machine perfusion with redox monitoring (Group II), or redox control (Group III). Control of the oxidation-reduction potential of the perfusate during machine perfusion of isolated hearts resulted in significantly improved systolic and diastolic function after perfusion compared to hearts that underwent machine perfusion without redox control or simple cold storage.
简单的冷藏仍然是目前人类心脏异体移植物在切除和移植之间的保存方法，但这种技术不足以进行长时间保存。冷藏的一种替代方法是对离体器官进行机器灌注。尽管该技术在肾脏保存方面已取得了有前景的结果，但在心脏方面的经验有限。限制灌注保存效果的一个因素是灌注过程中产生的氧自由基的毒性。这些自由基的产生和还原导致电子转移，产生一种称为氧化还原电位的电化学势。本研究探讨了在有或没有使用一种能够在灌注过程中控制氧化还原电位的新型电化学电池的情况下，机器灌注在心脏保存中的作用。心脏通过简单冷藏（第一组）、或通过有氧化还原监测的机器灌注（第二组）、或氧化还原控制（第三组）保存24小时。与未进行氧化还原控制的机器灌注或简单冷藏的心脏相比，在离体心脏的机器灌注过程中对灌注液的氧化还原电位进行控制，可使灌注后的心脏收缩和舒张功能得到显著改善。
