了解肠炎沙门氏菌血清型鸡白痢和鸡伤寒生物型的全球基因组特征。
Insights into the global genomic features of Salmonella enterica serovar Gallinarum biovars Gallinarum and Pullorum.
机构信息
Department of Pathology, Reproduction and One Health, School of Agriculture and Veterinarian Sciences, São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900 Jaboticabal, SP, Brazil.
Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Av. Prof. Dr. Zeferino Vaz, s/n, Campus da USP, CEP 14040-903 Ribeirão Preto, SP, Brazil.
出版信息
J Appl Microbiol. 2024 Sep 2;135(9). doi: 10.1093/jambio/lxae217.
AIMS
Characterize global genomic features of 86 genomes of Salmonella Gallinarum (SG) and Pullorum (SP), which are important pathogens causing systemic infections in poultry.
METHODS AND RESULTS
All genomes harbored efflux pump encoding gene mdsA and gold tolerance genes golS and golT. Aminoglycoside (aac(6')-Ib, aadA5, aph(6)-Id, aph(3'')-Ib, ant(2'')-Ia), beta-lactam (blaTEM-1, blaTEM-135), efflux pump (mdsB), fosfomycin (fosA3), sulfonamide (sul1, sul2), tetracycline [tet(A)], trimethoprim (dfrA17), acid (asr), and disinfectant (qacEdelta1) resistance genes, gyrA, gyrB, and parC quinolone resistance point mutations, and mercury tolerance genes (mer) were found in different frequencies. Additionally, 310 virulence genes, pathogenicity islands (including SPI-1, 2, 3, 4, 5, 6, 9, 10, 12, 13, and 14), plasmids [IncFII(S), ColpVC, IncX1, IncN, IncX2, and IncC], and prophages (Fels-2, ST104, 500465-1, pro483, Gifsy-2, 103 203_sal5, Fels-1, RE-2010, vB_SenS-Ent2, and L-413C) were detected. MLST showed biovar-specific sequence types, and core genome MLST showed country-specific and global-related clusters.
CONCLUSION
SG and SP global strains carry many virulence factors and important antimicrobial resistance genes. The diverse plasmids and prophages suggest genetic variability. MLST and cgMLST differentiated biovars and showed profiles occurring locally or worldwide.
目的
描述 86 株鸡白痢沙门氏菌（SG）和鸡伤寒沙门氏菌（SP）的全基因组特征，这两种菌是引起家禽全身感染的重要病原体。
方法和结果
所有基因组都携带了外排泵编码基因 mdsA 和金耐受基因 golS 和 golT。氨基糖苷类（aac(6')-Ib、aadA5、aph(6)-Id、aph(3'')-Ib、ant(2'')-Ia）、β-内酰胺类（blaTEM-1、blaTEM-135）、外排泵（mdsB）、磷霉素（fosA3）、磺胺类（sul1、sul2）、四环素 [tet(A)]、甲氧苄啶（dfrA17）、酸（asr）和消毒剂（qacEdelta1）耐药基因、gyrA、gyrB 和 parC 喹诺酮耐药点突变以及汞耐受基因（mer）的存在频率各不相同。此外，还检测到 310 种毒力基因、致病性岛（包括 SPI-1、2、3、4、5、6、9、10、12、13 和 14）、质粒[IncFII(S)、ColpVC、IncX1、IncN、IncX2 和 IncC]和原噬菌体（Fels-2、ST104、500465-1、pro483、Gifsy-2、103 203_sal5、Fels-1、RE-2010、vB_SenS-Ent2 和 L-413C）。MLST 显示了生物型特异性序列类型，核心基因组 MLST 显示了国家特异性和全球相关性聚类。
结论
SG 和 SP 全球菌株携带许多毒力因子和重要的抗菌药物耐药基因。多样化的质粒和原噬菌体表明存在遗传变异性。MLST 和 cgMLST 区分了生物型，并显示出在当地或全球范围内发生的特征。
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