基于全基因组测序对中国分离的鸡白痢沙门氏菌肠炎亚种菌株的耐药性和克隆关系分析。

Antimicrobial resistance and clonal relationships of Salmonella enterica Serovar Gallinarum biovar pullorum strains isolated in China based on whole genome sequencing.

机构信息

College of Animal Medicine, Shandong Agricultural University, Tai'an, 271018, Shandong, China.

China Animal Disease Control Center, Beijing, 102618, China.

出版信息

BMC Microbiol. 2024 Oct 18;24(1):414. doi: 10.1186/s12866-024-03296-3.

DOI:10.1186/s12866-024-03296-3

PMID:39425016

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11487782/

Abstract

BACKGROUND

Pullorum disease is a serious problem in many countries. Caused by Salmonella enterica serovar Gallinarum biovar Pullorum (S. Pullorum), it creates huge economic losses in the poultry industry. Although pullorum disease has been well-controlled in many developed countries, it is still a critical problem in developing countries. However, there is still a lack of information on S. Pullorum strains isolated from different regions and sources in China. The objective of this study was to supply the antimicrobial resistance patterns and clonal relationships of S. Pullorum from breeder chicken farms.

METHODS

In this study, a total of 114 S. Pullorum strains recovered from 11 provinces and municipalities in China between 2020 and 2021 were selected. These 114 S. Pullorum strains were analyzed using whole genome sequencing (WGS). Antimicrobial resistance (AMR) was tested both by genotypic prediction using the WGS method and using disc diffusion to assess phenotypic AMR.

RESULTS

These 114 sequenced S. Pullorum strains were divided into three sequence types (STs), the dominant STs was ST92 (104/114). Further core genome multi-locus sequence typing analysis indicated that 114 S. Pullorum strains may have a close relationship, which could be clonally transmitted among different provinces and municipalities. Our results showed a close relationship between the S. Pullorum strains found in different regions, indicating these strains may have been transmitted in China a long time ago. Nearly all S. Pullorum strains 94.74% (n = 108) were resistant to at least one antimicrobial class, and 35.96% of the examined Salmonella strains were considered multiple drug resistant.

CONCLUSION

Overall, this study showed that S. Pullorum strains in China have a close genetic relationship in terms of antimicrobial resistance, suggesting widespread clonal transmission.

摘要

背景

禽白痢是许多国家的一个严重问题。它是由肠炎沙门氏菌血清型鸡白痢亚种（S. Pullorum）引起的，给家禽业造成了巨大的经济损失。尽管禽白痢在许多发达国家得到了很好的控制，但它仍是发展中国家的一个关键问题。然而，中国不同地区和来源的 S. Pullorum 菌株的信息仍然缺乏。本研究的目的是提供来自种鸡场的 S. Pullorum 菌株的抗生素耐药模式和克隆关系。

方法

本研究共选择了 2020 年至 2021 年间从中国 11 个省和直辖市采集的 114 株 S. Pullorum 菌株。这些 S. Pullorum 菌株通过全基因组测序（WGS）进行分析。通过 WGS 方法进行基因型预测和药敏纸片扩散法评估表型 AMR 来检测抗生素耐药性（AMR）。

结果

这 114 株测序的 S. Pullorum 菌株被分为三个序列型（ST），优势 ST 是 ST92（104/114）。进一步的核心基因组多位点序列分型分析表明，114 株 S. Pullorum 菌株可能具有密切的关系，可以在不同的省和直辖市之间克隆传播。我们的结果表明，不同地区分离的 S. Pullorum 菌株之间存在密切的关系，表明这些菌株可能在很久以前就在中国传播。几乎所有的 S. Pullorum 菌株（94.74%，n=108）对至少一种抗菌药物类别具有耐药性，35.96%的被检沙门氏菌菌株被认为是多重耐药的。