Koyano Takayuki, Onishi Kaori, Matsuyama Makoto, Fukushima Masaki, Kume Kazunori
Division of Cell Biology, Shigei Medical Research Institute, Okayama, Okayama, Japan.
Division of Molecular Genetics, Shigei Medical Research Institute, Okayama, Okayama, Japan.
MicroPubl Biol. 2024 Aug 5;2024. doi: 10.17912/micropub.biology.001265. eCollection 2024.
The correct localization of proteins is linked to their cellular function. The Pkd2 localizes to the endoplasmic reticulum and plasma membrane. Here we investigate the behavior of Pkd2 in response to calcium. Pkd2-GFP, normally enriched at the cell ends, is reduced from the plasma membrane by CaCl addition, while cytoplasmic dots and free GFP are increased. This suggests that Pkd2 is internalized and degraded in response to extracellular CaCl . This internalization is partially suppressed by treatment with an Arp2/3 inhibitor, CK-666. Our data provide new insights into the relationship between Pkd2 internalization and calcium response.
蛋白质的正确定位与其细胞功能相关。多囊蛋白2（Pkd2）定位于内质网和质膜。在此，我们研究了Pkd2对钙的反应行为。正常情况下在细胞末端富集的Pkd2 - GFP，通过添加CaCl₂后从质膜上减少，而细胞质中的小点和游离的GFP增加。这表明Pkd2会响应细胞外CaCl₂而被内化并降解。用肌动蛋白相关蛋白2/3（Arp2/3）抑制剂CK - 666处理可部分抑制这种内化。我们的数据为Pkd2内化与钙反应之间的关系提供了新的见解。
