Department of Gastroenterology, Wenzhou Third Clinical Institute Affiliated to Wenzhou Medical University, Third Affiliated Hospital of Shanghai University, Wenzhou People's Hospital, Wenzhou, Zhejiang, China.
Eur J Gastroenterol Hepatol. 2024 Nov 1;36(11):1305-1313. doi: 10.1097/MEG.0000000000002836. Epub 2024 Aug 8.
The aim of this study is to systematically examine and compare the characteristics distinguishing colorectal adenomatous polyps from normal mucosal intestinal microbiota.
A total of 30 specimens were obtained from patients diagnosed with colorectal adenomatous polyps (adenoma group) who underwent endoscopic removal at Wenzhou People's Hospital between September 2021 and November 2021. Concurrently, 30 normal mucosal specimens were collected from patients without adenomatous polyps (control group). Subsequently, microbiome total DNA extraction was carried out, followed by PCR amplification targeting the V3-V4 region of the 16S rDNA. High-throughput sequencing was conducted using the Illumina MiSeq platform. Subsequent to sequencing, bioinformatics analysis was used to assess the diversity, composition, and functional aspects of the intestinal microbiota in both study groups.
A notable dissimilarity in the microbiota structure was identified, specifically within the transverse colon, between these two groups ( P < 0.05). Species composition analysis revealed that Escherichia , Fusobacterium , and Bacteroides were predominant bacteria in both groups, with Escherichia and Enterobacter displaying significant differences at the genera level between the control group and the adenoma group ( P < 0.05). Correlation analysis and functional prediction demonstrated substantial disparities in interactions among dominant intestinal microbial genera within patients from both groups. Additionally, it was discovered that the intestinal microbiomes in patients in the adenoma group exhibited a significantly higher pathogenic potential.
Upon conducting a comprehensive analysis, it was discerned that the microbiota present in the transverse colon of the control group exhibited distinctive characteristics that may contribute to the maintenance of intestinal health.
本研究旨在系统地检查和比较区分结直肠腺瘤性息肉与正常黏膜肠道微生物群的特征。
本研究共纳入 30 例 2021 年 9 月至 2021 年 11 月在温州市人民医院接受内镜下切除的结直肠腺瘤性息肉患者（腺瘤组）的标本，同时收集 30 例无腺瘤性息肉的正常黏膜患者（对照组）的标本。随后，提取微生物组总 DNA，然后对 16S rDNA 的 V3-V4 区进行 PCR 扩增。使用 Illumina MiSeq 平台进行高通量测序。测序后，使用生物信息学分析评估两组肠道微生物群的多样性、组成和功能。
两组患者横结肠内的微生物结构存在显著差异（P<0.05）。物种组成分析显示，两组均以大肠杆菌、梭杆菌属和拟杆菌属为主，对照组和腺瘤组在属水平上以大肠杆菌和肠杆菌的差异最为显著（P<0.05）。相关性分析和功能预测表明，两组患者主要肠道微生物属之间的相互作用存在显著差异。此外，还发现腺瘤组患者的肠道微生物组具有更高的致病性。
综合分析表明，对照组横结肠内的微生物群具有独特的特征，可能有助于维持肠道健康。
