Universidade Estadual de Feira de Santana, Departamento de Saúde, Laboratório de Fitoquímica, Rodovia Transnordestina, s/n, 44036-900 Feira de Santana, BA, Brazil.
Universidade Estadual do Sudoeste da Bahia, Departamento de Ciências Biológicas, Laboratório de Zoologia, Rua José Moreira Sobrinho, s/n, Jequiezinho, 45200-000 Jequié, BA, Brazil.
An Acad Bras Cienc. 2024 Aug 19;96(3):e20230493. doi: 10.1590/0001-3765202420230493. eCollection 2024.
Diplopods are terrestrial arthropods important for the dynamics of terrestrial ecosystems. One of the reasons for that can be their low predation rate due to their defensive secretion. Thus, Orthoporus fuscipes, a species belonging to this group and endemic to northeastern Brazil, was investigated as to its population structure and chemical constituents of defensive secretion. The population structure showed that females are larger and have greater mass than do males, along with negative allometric growth between males and females. The defensive secretion hexane extract was submitted to fractionation using SiO2 open-column chromatography and the gas chromatographic coupled to mass spectrometric analysis was applied in the fraction possibilities to identify major fatty acid methyl esthers, along with minor alkanes, alkenes and fatty acids derivatives and the known quinoids 2-methoxy-3-methylhydroquinone, 2-methoxy-3-methyl-1,4-benzoquinone, 2,3-dimethoxy-1,4-benzoquinone and 2,3-dimethoxyhydroquinone. In addition, the cytochrome oxidase I sequence for the species was deposited for the first time.
倍足纲动物是陆地节肢动物，对陆地生态系统的动态具有重要意义。其中一个原因可能是它们具有防御性分泌物，从而降低了被捕食的风险。因此，研究了属于这一组的巴西东北部特有物种Orthoporus fuscipes 的种群结构和防御性分泌物的化学成分。种群结构表明，雌性比雄性体型更大，质量也更大，而雄性和雌性之间的生长呈负异速生长。将防御性分泌物的己烷提取物用 SiO2 开管柱色谱法进行分离，然后在气相色谱-质谱联用分析中对各馏分进行分析，以确定主要脂肪酸甲酯，以及少量的烷烃、烯烃和脂肪酸衍生物，以及已知的醌类化合物 2-甲氧基-3-甲基氢醌、2-甲氧基-3-甲基-1,4-苯醌、2,3-二甲氧基-1,4-苯醌和 2,3-二甲氧基氢醌。此外，该物种的细胞色素氧化酶 I 序列也是首次被提交。
