Imam Abdulrahman Bin Faisal University, Department of Basic Science, Deanship of Preparatory Year and Supporting Studies, Dammam, Saudi Arabia.
Alexandria University, Faculty of Science, Department of Zoology, Alexandria, Egypt.
Braz J Biol. 2024 Aug 19;84:e282979. doi: 10.1590/1519-6984.282979. eCollection 2024.
The horned octopod Eledone cirrhosa, a medium-sized species found in Arabian Gulf off Saudi Arabia, was collected monthly from the Arabian Gulf off Dammam city during January to December 2022. Samples were dissected and prepared for examination using transmission electron microscopy. During genital maturation, seminiferous tubules are established in the testis, with active spermatogonia dividing. Spermatocytes 1 are observed in the tubule, followed by an increase in spermatogonia and spermatocytes in August. Spermiogenesis begins, with spherical spermatids evolving into elongated spermatids. In September, active spermatogonia, meiotic divisions, and increased spermiogenesis continue. Spermatozoa appear in Needham's pouch, indicating sexual maturity. The ovary undergoes various stages of development, with oocytes at stage I in June and July, followed by stage II in October and November. In stage III, follicular cords invade the oocyte's cytoplasm, forming numerous lipid inclusions and protein granules. The cytoplasm contains cisternae of endoplasmic reticulum and a poorly developed Golgi apparatus. Stage IV occurs in November, characterized by the maximum development of follicular cords and the beginning of vitellogenesis. The ooplasm contains numerous lipid inclusions, a syncytium, and secretory cells. From December, stage V oocytes are mainly present, indicating the activity phase of maximum secretion. Yolk platelets accumulate in the oocyte ooplasm, and chorion forms at the zona pellucida. In January, the first smooth eggs are found in some octopuses' ovary, with their proportion increasing steadily. This study aimed to investigate the mitogenic action of gonadotropin and identify the periods of intense cell multiplication during the sexual cycle using cytological methods.
阿拉伯湾沙特阿拉伯达曼市附近的 Horned octopod Eledone cirrhosa 是一种中型物种，2022 年 1 月至 12 月期间，每月从该地区采集样本。采集的样本经过解剖和准备，使用透射电子显微镜进行检查。在生殖成熟过程中，睾丸中建立了生精小管，活跃的精原细胞分裂。在小管中观察到精母细胞 1，随后在 8 月观察到精原细胞和精母细胞增加。精子发生开始，球形精子细胞演变为长形精子细胞。9 月，活跃的精原细胞、减数分裂和增加的精子发生继续进行。精子出现在 Needham 袋中，表明性成熟。卵巢经历了不同的发育阶段，6 月和 7 月的卵母细胞处于 I 期，10 月和 11 月的卵母细胞处于 II 期。在 III 期，滤泡索侵入卵母细胞的细胞质，形成许多脂质内含物和蛋白质颗粒。细胞质中含有内质网的池和发育不良的高尔基体。11 月发生 IV 期，特征是滤泡索的最大发育和卵黄发生的开始。卵质中含有许多脂质内含物、合胞体和分泌细胞。12 月起，主要出现 V 期卵母细胞，表明是最大分泌活性的阶段。卵黄小板在卵母细胞的卵质中积累，在透明带形成绒毛膜。1 月，在一些章鱼的卵巢中发现了第一批光滑的卵子，其比例稳步增加。本研究旨在使用细胞学方法研究促性腺激素的有丝分裂作用，并确定性周期中细胞增殖强烈的时期。
