College of Plant Protection, Southwest University, Chongqing, China.
Chongqing Key Laboratory of Soft-Matter Material Chemistry and Function Manufacturing, Southwest University, Chongqing, China.
Pest Manag Sci. 2024 Dec;80(12):6483-6492. doi: 10.1002/ps.8383. Epub 2024 Aug 21.
Pepper blight, caused by Phytophthora capsici, is a devastating disease that seriously threatens pepper production worldwide. With the emergence of resistance in P. capsici against conventional fungicides, there is an urgent need to explore novel alternatives for pepper blight management. This study aims to assess the inhibitory effect of chloroinconazide (CHI), a compound synthesized from tryptophan, against pepper blight, and to explore its potential mechanisms of action.
The results demonstrated that CHI effectively targeted P. capsici, disrupting its growth and mycelial structure, which resulted in the release of dissolved intracellular substances. Additionally, CHI significantly inhibited the sporangium formation, zoospores release, and zoospores germination, thereby reducing the re-infection of P. capsici. In contrast, the commercial pesticide methylaxyl only inhibited mycelial growth and had limited effect on re-infection, while azoxystrobin inhibited re-infection but had a weak inhibitory effect on mycelial growth. Furthermore, CHI activated the salicylic acid (SA) signaling pathway-mediated immune response to inhibit P. capsici infection in pepper, with this activation being contingent upon cyclic nucleotide-gated ion channel CaCNGC9.
CHI exhibited potent dual inhibitory effects on P. capsici by disrupting mycelial structure and activating the CaCNGC9-mediated SA signaling pathway. These dual mechanisms of action suggested that CHI could serve as a promising alternative chemical fungicide for the effective management of pepper blight, offering a new approach to control this devastating disease. Our findings highlighted the potential of CHI as a sustainable and efficient solution to combat the increasing resistance of P. capsici to conventional fungicides, ensuring better crop protection and yield. © 2024 Society of Chemical Industry.
辣椒疫霉引起的辣椒疫病是一种毁灭性疾病，严重威胁着全球辣椒生产。随着辣椒疫霉菌对常规杀菌剂产生抗性，迫切需要探索辣椒疫病管理的新替代方法。本研究旨在评估由色氨酸合成的化合物氯硝菌胺（CHI）对辣椒疫病的抑制作用，并探讨其潜在的作用机制。
结果表明，CHI 能有效靶向辣椒疫霉菌，破坏其生长和菌丝结构，导致细胞内溶解物质的释放。此外，CHI 显著抑制孢子囊形成、游动孢子释放和游动孢子萌发，从而减少辣椒疫霉菌的再感染。相比之下，商业农药甲基托布津仅抑制菌丝生长，对再感染的抑制作用有限，而肟菌酯抑制再感染，但对菌丝生长的抑制作用较弱。此外，CHI 通过激活水杨酸（SA）信号通路介导的免疫反应抑制辣椒疫霉菌对辣椒的感染，这种激活依赖于环核苷酸门控离子通道 CaCNGC9。
CHI 通过破坏菌丝结构和激活 CaCNGC9 介导的 SA 信号通路，对辣椒疫霉菌表现出强烈的双重抑制作用。这两种作用机制表明 CHI 可作为一种有前途的替代化学杀菌剂，有效防治辣椒疫病，为控制这种毁灭性病害提供了新的方法。我们的研究结果表明，CHI 作为一种可持续和有效的解决方案，具有对抗辣椒疫霉菌对常规杀菌剂日益增加的抗性的潜力，确保了更好的作物保护和产量。© 2024 化学工业协会。
