Shi Fengyan, Wang Xiuxue, Wei Meijun, Zhang Xi, Wang Zhidan, Lu Xiaochun, Zou Chunlei
Vegetable Research Institute, Liaoning Academy of Agricultural Sciences, Shenhe District, 84 Dongling Road, Shenyang, 110161, China.
College of Horticulture, Shenyang Agricultural University, Shenhe District, 120 Dongling Road, Shenyang, 110866, China.
BMC Genomics. 2025 Mar 29;26(1):311. doi: 10.1186/s12864-025-11498-w.
Phytophthora blight is a highly destructive soil-borne disease caused by Phytophthora capsici Leonian, which threatens pepper production. The molecular mechanism of pepper resistance to phytophthora blight is unclear, and the excavation and functional analysis of resistant genes are the bases and prerequisites for phytophthora blight-resistant breeding. We aimed to analyze the expression patterns of key genes in the plant-pathogen interaction metabolic pathway and propose a working model of the pepper defense signal network against Phytophthora capsici infection.
The 'ZCM334' pepper material used in this study is a high-generation inbred line that is immune to Phytophthora capsici and shows no signs of infection after inoculation. Comparative transcriptome analysis of the roots of 'ZCM334' and the susceptible material 'Early Calwonder' revealed significant differences in their gene expression profiles at different stages after inoculation. Most differentially expressed genes were significantly enriched in the biosynthesis of secondary metabolites, phenylpropanoid biosynthesis, plant-pathogen interaction, and fatty acid degradation metabolic pathways. Some defense genes and transcription factors significant in pepper resistance to phytophthora blight were identified, including PR1, RPP13, FLS2, CDPK, CML, MAPK, RLP, RLK, WRYK, ERF, MYB, and bHLH, most of which were regulated after inoculation. A working model was constructed for the defense signal network of pepper against Phytophthora capsici.
These data provide a valuable source of information for improving our understanding of the potential molecular mechanisms by which pepper plants resist infection by Phytophthora capsici. The identification of key genes and metabolic pathways provides avenues for further exploring the immune mechanism of 'ZCM334' resistance to phytophthora blight.
辣椒疫霉病是由辣椒疫霉（Phytophthora capsici Leonian）引起的一种极具破坏性的土传病害，威胁着辣椒生产。辣椒对疫霉病的抗性分子机制尚不清楚，抗性基因的挖掘和功能分析是辣椒抗疫霉病育种的基础和前提。本研究旨在分析植物-病原体互作代谢途径中关键基因的表达模式，并提出辣椒抵御辣椒疫霉感染的防御信号网络工作模型。
本研究中使用的“ZCM334”辣椒材料是一个高世代自交系，对辣椒疫霉免疫，接种后无感染迹象。对“ZCM334”和感病材料“Early Calwonder”的根部进行比较转录组分析，揭示了接种后不同阶段它们的基因表达谱存在显著差异。大多数差异表达基因在次生代谢物生物合成、苯丙烷生物合成、植物-病原体互作和脂肪酸降解代谢途径中显著富集。鉴定了一些在辣椒抗疫霉病中起重要作用的防御基因和转录因子，包括PR1、RPP13、FLS2、CDPK、CML、MAPK、RLP、RLK、WRYK、ERF、MYB和bHLH，其中大多数在接种后受到调控。构建了辣椒抵御辣椒疫霉的防御信号网络工作模型。
这些数据为增进我们对辣椒植株抵御辣椒疫霉感染潜在分子机制的理解提供了有价值的信息来源。关键基因和代谢途径的鉴定为进一步探索“ZCM334”抗疫霉病的免疫机制提供了途径。
