Quan Runze, Decraecker Lisse, Appeltans Iris, Cuende-Estévez María, Van Remoortel Samuel, Aguilera-Lizarraga Javier, Wang Zheng, Hicks Gareth, Wykosky Jill, McLean Peter, Denadai-Souza Alexandre, Hussein Hind, Boeckxstaens Guy E
Center for Intestinal Neuroimmune Interactions, Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven Department of Chronic Diseases and Metabolism (CHROMETA), KU Leuven, Leuven, Belgium.
Laboratory of Sensory Neurophysiology and Pain, Department of Pharmacology, University of Cambridge, Cambridge, UK.
Am J Gastroenterol. 2025 Mar 1;120(3):603-613. doi: 10.14309/ajg.0000000000003042. Epub 2024 Aug 21.
Changes in the composition of the gut microbiota have been associated with the development of irritable bowel syndrome (IBS). However, to what extent specific bacterial species relate to clinical symptoms remains poorly characterized. We investigated the clinical relevance of bacterial species linked with increased proteolytic activity, histamine production, and superantigen (SAg) production in patients with IBS.
Fecal (n = 309) and nasal (n = 214) samples were collected from patients with IBS and healthy volunteers (HV). Clinical symptoms and gut transit time were evaluated. Bacterial abundance in feces and nasal swabs as well as fecal trypsin-like activity were assessed.
The percentage of fecal samples containing Staphylococcus aureus was significantly higher in IBS compared with HV. Forty-nine percent of S. aureus -positive fecal samples from patients with IBS were also positive for SAgs, compared with 12% of HV. Patients with IBS and positive fecal SAg-producing S. aureus reported higher pain scores than those without S. aureus . Moreover, increased fecal proteolytic activity was associated with abdominal pain. Fecal abundance of Paraprevotella clara and Alistipes putredinis was significantly decreased in IBS, particularly in samples with higher proteolytic activity. Patients with lower Alistipes putredinis or Faecalibacterium prausnitzii abundance reported more severe abdominal pain.
In keeping with our preclinical findings, we show that increased presence of SAg-producing S. aureus in fecal samples of patients with IBS is associated with increased levels of abdominal pain. We also show that increased fecal proteolytic activity is associated with increased abdominal pain in patients with IBS.
肠道微生物群组成的变化与肠易激综合征（IBS）的发生有关。然而，特定细菌种类与临床症状的关联程度仍不清楚。我们研究了与IBS患者蛋白水解活性增加、组胺产生和超抗原（SAg）产生相关的细菌种类的临床相关性。
从IBS患者和健康志愿者（HV）中收集粪便（n = 309）和鼻腔（n = 214）样本。评估临床症状和肠道转运时间。评估粪便和鼻拭子中的细菌丰度以及粪便胰蛋白酶样活性。
与HV相比，IBS患者粪便中含有金黄色葡萄球菌的样本百分比显著更高。IBS患者49%的金黄色葡萄球菌阳性粪便样本SAg也呈阳性，而HV中这一比例为12%。IBS且粪便中产生SAg的金黄色葡萄球菌呈阳性的患者疼痛评分高于无金黄色葡萄球菌的患者。此外，粪便蛋白水解活性增加与腹痛有关。IBS患者中，清晰副普雷沃菌和腐败阿利斯杆菌的粪便丰度显著降低，尤其是在蛋白水解活性较高的样本中。腐败阿利斯杆菌或普拉梭菌丰度较低的患者腹痛更严重。
与我们的临床前研究结果一致，我们发现IBS患者粪便样本中产生SAg的金黄色葡萄球菌增加与腹痛水平升高有关。我们还表明，粪便蛋白水解活性增加与IBS患者腹痛增加有关。
